Development of CRISPR-CAS9 based RNA drugs against Eimeria tenella infection.

Animals CRISPR-Cas Systems Chickens / genetics Eimeria tenella / genetics RNA RNA, Messenger / genetics
CRISPR-Cas9 DNA barcode E. tenella Quantitative real-time PCR Species-specific anti-parasitic drug Target gene sequencing

Journal

Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135

Informations de publication

Date de publication:
11 2021
Historique:
received: 10 07 2021
revised: 16 09 2021
accepted: 31 10 2021
pubmed: 7 11 2021
medline: 1 4 2022
entrez: 6 11 2021
Statut: ppublish

Résumé

Parasitic diseases are major trouble in many parts of the world. We consider that if a chemical can break a DNA barcode sequence, it might be used to develop a species-specific anti-parasitic agent. To examine this hypothesis, we constructed sgRNAs that target both the control (5.8S rDNA) and a DNA barcode (ITS) sequence in Eimeria tenella. In vitro experiment showed that Cas9 mRNA combined with sgRNAs could reduce the sporulation percentage of oocysts and the survival rate of sporulated oocysts and sporozoites. Quantitative real-time PCR showed that the DNAs of parasites exposed to Cas9 mRNA and sgRNAs were significantly affected, regardless of whether they were exposed to a combination of two sgRNAs or just a single sgRNA. The DNA sequencing also indicated that the experimental group exposed to two sgRNAs mixed with Cas9-induced deletion of large parts and a single sgRNA mixed with Cas9-induced mutation at sgRNA targeted fragments. In vivo trial, the effect of sgRNA and Cas9 RNA on the pathogenicity of E. tenella in chicken showed less lesion score and oocysts score (P < 0.05) in experimental groups than control groups. The results and concepts presented in this research can lead to discovering novel nucleic acid therapeutic drugs for Eimeriasis and other parasitic infections, which provide insights into the development of species-specific anti-parasitic agents.

Identifiants

DOI: 10.1016/j.ygeno.2021.10.019 PMID: 34740777
pubmed: 34740777
pii: S0888-7543(21)00382-7
doi: 10.1016/j.ygeno.2021.10.019
pii:
doi:

Substances chimiques

RNA, Messenger 0
RNA 63231-63-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4126-4135

Informations de copyright

Copyright © 2021. Published by Elsevier Inc.

Auteurs

Muhammad Mohsin (M)

College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; Department of Parasitology, University of Agriculture, Faisalabad, Pakistan.

Yige Li (Y)

College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.

Xin Zhang (X)

College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.

Yilei Wang (Y)

College of Fisheries, Jimei University, Xiamen, Fujian, China.

Zhijian Huang (Z)

College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

Guangwen Yin (G)

College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China. Electronic address: yinguangwen000@sina.com.

Ziping Zhang (Z)

College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China. Electronic address: zhangziping@hotmail.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Development of CRISPR-CAS9 based RNA drugs...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Development of CRISPR-CAS9 based RNA drugs...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Poulets › Development of CRISPR-CAS9 based RNA drugs...
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Coccidia › Eimeriida › Eimeriidae › Eimeria › Eimeria tenella › Development of CRISPR-CAS9 based RNA drugs...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Development of CRISPR-CAS9 based RNA drugs...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Development of CRISPR-CAS9 based RNA drugs...