Bi-allelic loss-of-function variants in


Journal

Journal of medical genetics
ISSN: 1468-6244
Titre abrégé: J Med Genet
Pays: England
ID NLM: 2985087R

Informations de publication

Date de publication:
01 2023
Historique:
received: 27 06 2021
accepted: 14 10 2021
pubmed: 7 11 2021
medline: 28 12 2022
entrez: 6 11 2021
Statut: ppublish

Résumé

Fetal akinesia (FA) results in variable clinical presentations and has been associated with more than 166 different disease loci. However, the underlying molecular cause remains unclear in many individuals. We aimed to further define the set of genes involved. We performed in-depth clinical characterisation and exome sequencing on a cohort of 23 FA index cases sharing arthrogryposis as a common feature. We identified likely pathogenic or pathogenic variants in 12 different established disease genes explaining the disease phenotype in 13 index cases and report 12 novel variants. In the unsolved families, a search for recessive-type variants affecting the same gene was performed; and in five affected fetuses of two unrelated families, a homozygous loss-of-function variant in the Our study underlines the broad locus heterogeneity of FA with well-established and atypical genotype-phenotype associations. We describe

Sections du résumé

BACKGROUND
Fetal akinesia (FA) results in variable clinical presentations and has been associated with more than 166 different disease loci. However, the underlying molecular cause remains unclear in many individuals. We aimed to further define the set of genes involved.
METHODS
We performed in-depth clinical characterisation and exome sequencing on a cohort of 23 FA index cases sharing arthrogryposis as a common feature.
RESULTS
We identified likely pathogenic or pathogenic variants in 12 different established disease genes explaining the disease phenotype in 13 index cases and report 12 novel variants. In the unsolved families, a search for recessive-type variants affecting the same gene was performed; and in five affected fetuses of two unrelated families, a homozygous loss-of-function variant in the
CONCLUSION
Our study underlines the broad locus heterogeneity of FA with well-established and atypical genotype-phenotype associations. We describe

Identifiants

pubmed: 34740919
pii: jmedgenet-2021-108064
doi: 10.1136/jmedgenet-2021-108064
pmc: PMC9811090
doi:

Substances chimiques

KIF21A protein, human 0
Kinesins EC 3.6.4.4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

48-56

Informations de copyright

© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

Déclaration de conflit d'intérêts

Competing interests: None declared.

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Auteurs

Ruth J Falb (RJ)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Amelie J Müller (AJ)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany Amelie.Mueller@med.uni-tuebingen.de.

Wolfram Klein (W)

genetikum Stuttgart, Stuttgart, Germany.

Mona Grimmel (M)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Ute Grasshoff (U)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Stephanie Spranger (S)

Practice of Human Genetics, Bremen, Germany.

Petra Stöbe (P)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Darja Gauck (D)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Alma Kuechler (A)

Institute of Human Genetics, University Hospital Essen, Essen, Germany.

Nicola Dikow (N)

Institute of Human Genetics, Heidelberg University, Heidelberg, Germany.

Eva M C Schwaibold (EMC)

Institute of Human Genetics, Heidelberg University, Heidelberg, Germany.

Christoph Schmidt (C)

genetikum Neu-Ulm, Neu-Ulm, Germany.

Luisa Averdunk (L)

Department of General Pediatrics, Neonatology and Pediatric Cardiology, Heinrich-Heine-University Dusseldorf, Dusseldorf, Germany.

Rebecca Buchert (R)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Tilman Heinrich (T)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Natalia Prodan (N)

Department of Women's Health, University Women's Hospital, Tuebingen, Germany.

Joohyun Park (J)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Martin Kehrer (M)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Marc Sturm (M)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Olga Kelemen (O)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Silke Hartmann (S)

genetikum Neu-Ulm, Neu-Ulm, Germany.

Denise Horn (D)

Institute of Medical and Human Genetics, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Dirk Emmerich (D)

Practice for Ultrasound and Prenatal Medicine, Freiburg, Germany.

Nina Hirt (N)

Institute of Human Genetics, University Medical Center Freiburg, Freiburg, Germany.

Armin Neumann (A)

Practice for Prenatal Medicine, Bremen, Germany.

Glen Kristiansen (G)

Institute of Pathology, Center for Integrated Oncology, University of Bonn, Bonn, Germany.

Ulrich Gembruch (U)

Department of Obstetrics and Prenatal Medicine, University Hospital Bonn, Bonn, Germany.

Susanne Haen (S)

Institute of Pathology and Neuropathology, University of Tuebingen, Tuebingen, Germany.

Reiner Siebert (R)

Institute of Human Genetics, Ulm University and Ulm University Medical Center, Ulm, Germany.

Sabine Hentze (S)

Practice for Human Genetics, Heidelberg, Germany.

Markus Hoopmann (M)

Department of Women's Health, University Women's Hospital, Tuebingen, Germany.

Stephan Ossowski (S)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Stephan Waldmüller (S)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Stefanie Beck-Wödl (S)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.

Dieter Gläser (D)

genetikum Neu-Ulm, Neu-Ulm, Germany.

Ismail Tekesin (I)

Prenatal Medicine Stuttgart, Stuttgart, Germany.

Felix Distelmaier (F)

Department of General Pediatrics, Neonatology and Pediatric Cardiology, Heinrich-Heine-University Dusseldorf, Dusseldorf, Germany.

Olaf Riess (O)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.
Centre for Rare Diseases, University of Tuebingen, Tuebingen, Germany.

Karl-Oliver Kagan (KO)

Department of Women's Health, University Women's Hospital, Tuebingen, Germany.

Andreas Dufke (A)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.
Centre for Rare Diseases, University of Tuebingen, Tuebingen, Germany.

Tobias B Haack (TB)

Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Tuebingen, Germany.
Centre for Rare Diseases, University of Tuebingen, Tuebingen, Germany.

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