A comparative study on the secretion of various cytokines by pulp stem cells at different passages and their neurogenic potential.
brain-derived neurotrophic factor
cytokines
dental pulp stem cell
multidirectional differentiation
nerve growth factor
neurotrophin-3. immunophenotype
vascular endothelial growth factor
Journal
Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
ISSN: 1804-7521
Titre abrégé: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
Pays: Czech Republic
ID NLM: 101140142
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
received:
22
06
2021
accepted:
01
10
2021
pubmed:
9
11
2021
medline:
20
5
2022
entrez:
8
11
2021
Statut:
ppublish
Résumé
By measuring the extent of cytokines secreted by human dental pulp stem cells (hDPSCs) from passages 2 through 10, the optimal passage of hDPSCs was determined. This offers a potential theoretical basis for the treatment of neurological disorders. After isolation and culture of hDPSCs from human teeth, the morphological features of the cells were observed under an inverted microscope. hDPSCs were identified by their immunophenotypes and their multiple differentiation capability. Cytokine concentrations secreted in the supernatants at passages 2-10 were detected by ELISA. hDPSCs were viewed as fusiform or polygonal in shape, with a bulging cell body, homogenized cytoplasm, and a clear nucleus. Moreover, they could differentiate into neuroblasts in vitro. hDPSCs at passage 3 were positive for CD29 (91.5%), CD73 (94.8%) and CD90 (96.7%), but negative for the hematopoietic markers CD34 (0.13%). ELISA results showed that hDPSCs at passage 3 had the highest secretion levels of vascular endothelial growth factor (VEGF), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF), with the highest secretion level of Neurotrophin-3 (NT-3) being at passage 2. hDPSCs have steady biological features of stem cells and exhibit optimal proliferation potential. hDPSCs at different passages have different capacities in the secretion of VEGF, BDNF, NGF, and NT-3. In conclusion cytokines secreted by hDPSCs may prove to be appropriate in the treatment of neurological diseases.
Substances chimiques
Brain-Derived Neurotrophic Factor
0
Cytokines
0
NTF3 protein, human
0
Neurotrophin 3
0
Vascular Endothelial Growth Factor A
0
Nerve Growth Factor
9061-61-4
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
161-167Déclaration de conflit d'intérêts
The authors report no conflicts of interest in this work.
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