The human telomeric proteome during telomere replication.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
02 12 2021
02 12 2021
Historique:
accepted:
13
10
2021
revised:
07
10
2021
received:
24
08
2021
pubmed:
9
11
2021
medline:
11
1
2022
entrez:
8
11
2021
Statut:
ppublish
Résumé
Telomere shortening can cause detrimental diseases and contribute to aging. It occurs due to the end replication problem in cells lacking telomerase. Furthermore, recent studies revealed that telomere shortening can be attributed to difficulties of the semi-conservative DNA replication machinery to replicate the bulk of telomeric DNA repeats. To investigate telomere replication in a comprehensive manner, we develop QTIP-iPOND - Quantitative Telomeric chromatin Isolation Protocol followed by isolation of Proteins On Nascent DNA - which enables purification of proteins that associate with telomeres specifically during replication. In addition to the core replisome, we identify a large number of proteins that specifically associate with telomere replication forks. Depletion of several of these proteins induces telomere fragility validating their importance for telomere replication. We also find that at telomere replication forks the single strand telomere binding protein POT1 is depleted, whereas histone H1 is enriched. Our work reveals the dynamic changes of the telomeric proteome during replication, providing a valuable resource of telomere replication proteins. To our knowledge, this is the first study that examines the replisome at a specific region of the genome.
Identifiants
pubmed: 34747482
pii: 6423196
doi: 10.1093/nar/gkab1015
pmc: PMC8643687
doi:
Substances chimiques
Histones
0
POT1 protein, human
0
Shelterin Complex
0
Telomere-Binding Proteins
0
Telomerase
EC 2.7.7.49
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12119-12135Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Cell. 2012 Aug 31;150(5):922-33
pubmed: 22921915
Science. 2015 Jan 23;347(6220):1260419
pubmed: 25613900
Nat Commun. 2013;4:2848
pubmed: 24270157
Br J Haematol. 2015 Aug;170(4):457-71
pubmed: 25940403
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Mol Biol Cell. 2006 Feb;17(2):944-54
pubmed: 16319170
Science. 2007 Nov 2;318(5851):798-801
pubmed: 17916692
BMC Cell Biol. 2003 Oct 27;4:15
pubmed: 14577841
Mol Cell. 2018 May 3;70(3):395-407.e4
pubmed: 29727616
Nat Commun. 2010 Jun 29;1:33
pubmed: 20975687
Cell. 2016 Aug 25;166(5):1188-1197.e9
pubmed: 27523609
Nat Struct Mol Biol. 2015 Nov;22(11):867-74
pubmed: 26581521
Cell. 2001 Nov 2;107(3):347-59
pubmed: 11701125
Biomolecules. 2015 Jul 17;5(3):1515-39
pubmed: 26193331
J Mol Biol. 2009 May 22;388(5):928-40
pubmed: 19265708
J Cell Biol. 2009 Jun 1;185(5):827-39
pubmed: 19487455
Epigenomics. 2019 Feb;11(4):363-366
pubmed: 30793938
EMBO Mol Med. 2019 Jul;11(7):e10201
pubmed: 31273937
Nucleic Acids Res. 2013 Apr;41(7):4026-35
pubmed: 23435226
Cell Rep. 2016 Jun 7;15(10):2170-2184
pubmed: 27239034
Methods. 2017 Feb 1;114:28-38
pubmed: 27520492
Nature. 2020 Nov;587(7833):303-308
pubmed: 33057192
EMBO J. 2020 Dec 1;39(23):e104500
pubmed: 33073402
Nat Commun. 2018 Feb 8;9(1):586
pubmed: 29422503
Cell Rep. 2020 Nov 10;33(6):108379
pubmed: 33176153
Genes Dev. 2020 Oct 1;34(19-20):1392-1405
pubmed: 32883681
J Cell Sci. 2013 Mar 15;126(Pt 6):1323-32
pubmed: 23378026
J Am Soc Mass Spectrom. 2011 Aug;22(8):1373-80
pubmed: 21953191
Mol Cell. 2009 Sep 11;35(5):642-56
pubmed: 19748358
Cell Rep. 2019 Sep 24;28(13):3497-3509.e4
pubmed: 31553917
Biochem Biophys Res Commun. 2001 Feb 2;280(4):961-3
pubmed: 11162618
EMBO J. 2013 Oct 30;32(21):2861-71
pubmed: 24084588
Cell Rep. 2014 May 22;7(4):1320-32
pubmed: 24813883
Nature. 2006 Apr 6;440(7085):824-8
pubmed: 16598261
Mol Cell Biol. 1994 Sep;14(9):5777-85
pubmed: 8065312
Cell. 2009 Jan 9;136(1):175-86
pubmed: 19135898
J Cell Biol. 2015 Jul 20;210(2):191-208
pubmed: 26195664
Cell Death Discov. 2017 Sep 04;3:17043
pubmed: 28875039
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Science. 2004 Dec 10;306(5703):1951-3
pubmed: 15591207
J Proteome Res. 2011 Apr 1;10(4):1794-805
pubmed: 21254760
Nat Commun. 2019 May 28;10(1):2253
pubmed: 31138795
Genes Dev. 2017 Mar 15;31(6):603-616
pubmed: 28404631
Nat Methods. 2013 Aug;10(8):730-6
pubmed: 23921808
Nature. 2004 Nov 18;432(7015):353-60
pubmed: 15525938
Genes Dev. 2005 Jul 15;19(14):1668-73
pubmed: 16024656
Nat Commun. 2018 Feb 8;9(1):557
pubmed: 29422501
Nature. 2011 Mar 24;471(7339):532-6
pubmed: 21399625
Cell. 2009 Jul 10;138(1):90-103
pubmed: 19596237
J Proteome Res. 2011 Mar 4;10(3):1216-27
pubmed: 21210717
Trends Cell Biol. 2015 Jan;25(1):29-36
pubmed: 25257515
Cell Rep. 2013 Apr 25;3(4):1105-16
pubmed: 23545495
Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3408-16
pubmed: 23959892
Nat Cell Biol. 2011 Jun;13(6):668-75
pubmed: 21532585
Nat Protoc. 2007;2(8):1896-906
pubmed: 17703201
Annu Rev Genomics Hum Genet. 2009;10:45-61
pubmed: 19405848
Science. 2017 Nov 10;358(6364):797-802
pubmed: 29123070
Life Sci Alliance. 2018 Jul 17;1(4):e201800121
pubmed: 30456372
Mol Cell. 2020 Jul 2;79(1):115-126.e6
pubmed: 32497497
J Clin Invest. 2015 May;125(5):2151-60
pubmed: 25893599
Cell. 2012 May 11;149(4):795-806
pubmed: 22579284
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
J Cell Sci. 2011 Nov 1;124(Pt 21):3706-14
pubmed: 22045740
Nat Commun. 2014 Oct 21;5:5220
pubmed: 25330849
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
Nat Commun. 2014 Oct 31;5:5379
pubmed: 25359189
Nat Cell Biol. 2014 Mar;16(3):281-93
pubmed: 24561620
J Cell Biol. 2007 Sep 24;178(7):1101-8
pubmed: 17893239
J Biol Chem. 2013 Nov 1;288(44):31458-67
pubmed: 24047897
Nat Protoc. 2012 Mar 01;7(3):594-605
pubmed: 22383038
EMBO J. 2006 Feb 8;25(3):565-74
pubmed: 16424902
Cell. 1993 May 21;73(4):775-87
pubmed: 8500170
Biochim Biophys Acta Gen Subj. 2018 Sep;1862(9):1973-1979
pubmed: 29906500
Mol Cell. 2018 Oct 18;72(2):239-249.e5
pubmed: 30146316
Genes Dev. 2016 Jul 1;30(13):1483-91
pubmed: 27401551
PLoS One. 2012;7(4):e34386
pubmed: 22536324
FEBS J. 2013 Jul;280(14):3180-93
pubmed: 23647631
Plant Cell. 2010 Aug;22(8):2768-80
pubmed: 20699390
Mol Cell. 2008 Nov 21;32(4):465-77
pubmed: 19026778
Bioessays. 2021 Oct;43(10):e2100157
pubmed: 34436787
Cell. 1999 May 14;97(4):503-14
pubmed: 10338214
Nat Rev Mol Cell Biol. 2017 Mar;18(3):175-186
pubmed: 28096526
BMC Genomics. 2016 Oct 17;17(Suppl 9):749
pubmed: 27766953
Cell Cycle. 2021 Sep;20(18):1745-1759
pubmed: 34432566
RNA. 2019 Nov;25(11):1470-1480
pubmed: 31350341
Mol Cell Biol. 2006 Dec;26(24):9279-90
pubmed: 17000763
Nucleic Acids Res. 2019 Jan 8;47(D1):D330-D338
pubmed: 30395331
J Biol Chem. 2009 Jan 30;284(5):2823-2829
pubmed: 19015270
Cell. 2008 May 16;133(4):627-39
pubmed: 18485871
Genes Dev. 2014 Nov 15;28(22):2477-91
pubmed: 25344324
J Biol Chem. 2014 Mar 28;289(13):8916-30
pubmed: 24526689
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Nat Commun. 2019 Mar 1;10(1):1001
pubmed: 30824709
Dev Cell. 2018 Feb 5;44(3):281-283
pubmed: 29408234
Cell Rep. 2018 Jun 5;23(10):3031-3041
pubmed: 29874588
Methods Enzymol. 2017;591:33-53
pubmed: 28645376
Mol Cell Biol. 2010 Oct;30(20):4808-17
pubmed: 20713443
Nat Struct Mol Biol. 2013 Oct;20(10):1199-205
pubmed: 24013207
Sci Rep. 2019 Dec 13;9(1):19110
pubmed: 31836759
Trends Biochem Sci. 2017 Oct;42(10):788-798
pubmed: 28870425
Nature. 2019 Nov;575(7783):523-527
pubmed: 31723267
Nat Commun. 2015 Jan 22;6:6138
pubmed: 25609380
Nature. 2003 Nov 13;426(6963):194-8
pubmed: 14608368
Annu Rev Genet. 2018 Nov 23;52:223-247
pubmed: 30208292
Mol Biol Cell. 2006 Feb;17(2):955-65
pubmed: 16339074
Nat Genet. 2015 Dec;47(12):1482-8
pubmed: 26482878