Inhibition of porcine epidemic diarrhea virus by Alpiniae oxyphyllae fructus polysaccharide 3.

Animals Cell Line Epithelial Cells Polysaccharides / pharmacology Porcine epidemic diarrhea virus Spectroscopy, Fourier Transform Infrared / veterinary Swine
Alpiniae oxyphyllae fructus polysaccharide 3 Porcine epidemic diarrhea virus Structural characterization

Journal

Research in veterinary science
ISSN: 1532-2661
Titre abrégé: Res Vet Sci
Pays: England
ID NLM: 0401300

Informations de publication

Date de publication:
Dec 2021
Historique:
received: 26 07 2021
revised: 23 10 2021
accepted: 30 10 2021
pubmed: 9 11 2021
medline: 15 12 2021
entrez: 8 11 2021
Statut: ppublish

Résumé

Porcine epidemic diarrhea virus (PEDV) is a deadly pathogen that still plagues suckling piglets. However, there is still no anti-PEDV drug available in clinics. To develop potential anti-PEDV drugs, the antiviral activity of Alpiniae oxyphyllae fructus polysaccharide 3 (AOFP3) against PEDV infection in IPEC-J2 cells were assessed in our present study. The structural characterization of AOFP3 was studied by using HPAEC, GC-MS, FT-IR and NMR techniques. At the same time, the anti-PEDV activity of AOFP3 was investigated by performing RT-qPCR, Western blot and immunofluorescence assays. The results showed that AOFP3 (44.4 kDa) was composed of glucose and galacturonic acid at a molar ratio of 77.54:22.46 and consisted of →4)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, T-α-D-Glcp-(1→ and →4)-α-D-GalAp-(1→. AOFP3 significantly decreased PEDV titer in IPEC-J2 cells and prevented cellular damage of IPEC-J2 cells caused by PEDV infection. Furthermore, AOFP3 showed an antioxidative activity in inhibiting PEDV reproduction. Therefore, AOFP3 was expected to be a material of anti-PEDV drug.

Identifiants

DOI: 10.1016/j.rvsc.2021.10.026 PMID: 34749099
pubmed: 34749099
pii: S0034-5288(21)00320-9
doi: 10.1016/j.rvsc.2021.10.026
pii:
doi:

Substances chimiques

Polysaccharides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

146-155

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Yun Chen (Y)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China. Electronic address: ychen@hainanu.edu.cn.

Yu Zhang (Y)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.

Qiyuan Luo (Q)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.

Yongjian Zhu (Y)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.

Huijun Du (H)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.

Suya Liao (S)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.

Yuhui Yang (Y)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.

Huricha Chen (H)

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou 570228, PR China.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Inhibition of porcine epidemic diarrhea virus...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Inhibition of porcine epidemic diarrhea virus...
questionsmedicales.fr › Cellules › Cellules épithéliales › Inhibition of porcine epidemic diarrhea virus...
questionsmedicales.fr › Glucides › Polyosides › Inhibition of porcine epidemic diarrhea virus...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Alphacoronavirus › Virus de la diarrhée porcine épidémique › Inhibition of porcine epidemic diarrhea virus...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrophotométrie › Spectrophotométrie IR › Spectroscopie infrarouge à transformée de Fourier › Inhibition of porcine epidemic diarrhea virus...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Inhibition of porcine epidemic diarrhea virus...