Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc.
Animals
Animals, Genetically Modified
Body Patterning
/ genetics
Bone Morphogenetic Proteins
/ genetics
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Gene Expression Regulation, Developmental
Imaginal Discs
/ growth & development
Microscopy, Confocal
Mutation
Signal Transduction
/ genetics
Wings, Animal
/ growth & development
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 11 2021
08 11 2021
Historique:
received:
16
11
2020
accepted:
20
10
2021
entrez:
9
11
2021
pubmed:
10
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
How morphogen gradients control patterning and growth in developing tissues remains largely unknown due to lack of tools manipulating morphogen gradients. Here, we generate two membrane-tethered protein binders that manipulate different aspects of Decapentaplegic (Dpp), a morphogen required for overall patterning and growth of the Drosophila wing. One is "HA trap" based on a single-chain variable fragment (scFv) against the HA tag that traps HA-Dpp to mainly block its dispersal, the other is "Dpp trap" based on a Designed Ankyrin Repeat Protein (DARPin) against Dpp that traps Dpp to block both its dispersal and signaling. Using these tools, we found that, while posterior patterning and growth require Dpp dispersal, anterior patterning and growth largely proceed without Dpp dispersal. We show that dpp transcriptional refinement from an initially uniform to a localized expression and persistent signaling in transient dpp source cells render the anterior compartment robust against the absence of Dpp dispersal. Furthermore, despite a critical requirement of dpp for the overall wing growth, neither Dpp dispersal nor direct signaling is critical for lateral wing growth after wing pouch specification. These results challenge the long-standing dogma that Dpp dispersal is strictly required to control and coordinate overall wing patterning and growth.
Identifiants
pubmed: 34750371
doi: 10.1038/s41467-021-26726-6
pii: 10.1038/s41467-021-26726-6
pmc: PMC8576045
doi:
Substances chimiques
Bone Morphogenetic Proteins
0
Drosophila Proteins
0
dpp protein, Drosophila
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6435Subventions
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2021. The Author(s).
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