Toxicity of stainless and mild steel particles generated from gas-metal arc welding in primary human small airway epithelial cells.
Air Pollutants, Occupational
/ chemistry
Animals
Cell Line
Cell Survival
/ drug effects
Cells, Cultured
Epithelial Cells
/ drug effects
Humans
Inhalation Exposure
/ adverse effects
Lung
/ drug effects
Mice
Microscopy, Electron, Transmission
Mouse Embryonic Stem Cells
/ drug effects
Particle Size
Reactive Oxygen Species
/ metabolism
Stainless Steel
/ chemistry
Steel
/ chemistry
Welding
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
08 11 2021
08 11 2021
Historique:
received:
30
06
2021
accepted:
21
10
2021
entrez:
9
11
2021
pubmed:
10
11
2021
medline:
27
1
2022
Statut:
epublish
Résumé
Welding fumes induce lung toxicity and are carcinogenic to humans but the molecular mechanisms have yet to be clarified. The aim of this study was to evaluate the toxicity of stainless and mild steel particles generated via gas-metal arc welding using primary human small airway epithelial cells (hSAEC) and ToxTracker reporter murine stem cells, which track activation of six cancer-related pathways. Metal content (Fe, Mn, Ni, Cr) of the particles was relatively homogenous across particle size. The particles were not cytotoxic in reporter stem cells but stainless steel particles activated the Nrf2-dependent oxidative stress pathway. In hSAEC, both particle types induced time- and dose-dependent cytotoxicity, and stainless steel particles also increased generation of reactive oxygen species. The cellular metal content was higher for hSAEC compared to the reporter stem cells exposed to the same nominal dose. This was, in part, related to differences in particle agglomeration/sedimentation in the different cell media. Overall, our study showed differences in cytotoxicity and activation of cancer-related pathways between stainless and mild steel welding particles. Moreover, our data emphasizes the need for careful assessment of the cellular dose when comparing studies using different in vitro models.
Identifiants
pubmed: 34750422
doi: 10.1038/s41598-021-01177-7
pii: 10.1038/s41598-021-01177-7
pmc: PMC8575907
doi:
Substances chimiques
Air Pollutants, Occupational
0
Reactive Oxygen Species
0
Stainless Steel
12597-68-1
Steel
12597-69-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21846Subventions
Organisme : AFA Försäkring
ID : 160226
Organisme : VR-RFI
ID : 2017-00646_9
Organisme : Stiftelsen för Strategisk Forskning
ID : RIF14-0053
Organisme : Forskningsrådet om Hälsa, Arbetsliv och Välfärd
ID : 2017-00690
Organisme : Forskningsrådet om Hälsa, Arbetsliv och Välfärd
ID : 2017-00252
Informations de copyright
© 2021. The Author(s).
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