Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus.
Animals
Bacterial Proteins
/ genetics
Biofilms
/ growth & development
Endoribonucleases
/ genetics
Flagella
/ ultrastructure
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Genes, Bacterial
Mice
Movement
RNA, Bacterial
/ genetics
RNA, Messenger
/ genetics
Vibrio Infections
/ microbiology
Vibrio vulnificus
/ enzymology
Virulence
Virulence Factors
/ genetics
RNase E
Vibrio vulnificus
VvRraA1
pathogenicity
virulence
Journal
Journal of microbiology (Seoul, Korea)
ISSN: 1976-3794
Titre abrégé: J Microbiol
Pays: Korea (South)
ID NLM: 9703165
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
05
10
2021
accepted:
20
10
2021
revised:
19
10
2021
pubmed:
10
11
2021
medline:
19
1
2022
entrez:
9
11
2021
Statut:
ppublish
Résumé
RraA, a protein regulator of RNase E activity, plays a unique role in modulating the mRNA abundance in Escherichia coli. The marine pathogenic bacterium Vibrio vulnificus also possesses homologs of RNase E (VvRNase E) and RraA (VvRraA1 and VvRraA2). However, their physiological roles have not yet been investigated. In this study, we demonstrated that VvRraA1 expression levels affect the pathogenicity of V. vulnificus. Compared to the wild-type strain, the VvrraA1-deleted strain (ΔVvrraA1) showed decreased motility, invasiveness, biofilm formation ability as well as virulence in mice; these phenotypic changes of ΔVvrraA1 were restored by the exogenous expression of VvrraA1. Transcriptomic analysis indicated that VvRraA1 expression levels affect the abundance of a large number of mRNA species. Among them, the half-lives of mRNA species encoding virulence factors (e.g., smcR and htpG) that have been previously shown to affect VvrraA1 expression-dependent phenotypes were positively correlated with VvrraA1 expression levels. These findings suggest that VvRraA1 modulates the pathogenicity of V. vulnificus by regulating the abundance of a subset of mRNA species.
Identifiants
pubmed: 34751908
doi: 10.1007/s12275-021-1518-5
pii: 10.1007/s12275-021-1518-5
doi:
Substances chimiques
Bacterial Proteins
0
RNA, Bacterial
0
RNA, Messenger
0
Virulence Factors
0
Endoribonucleases
EC 3.1.-
ribonuclease E
EC 3.1.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1133-1141Informations de copyright
© 2021. The Microbiological Society of Korea.
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