Characterization of a P-glycoprotein drug transporter from Toxocara canis with a novel pharmacological profile.


Journal

International journal for parasitology. Drugs and drug resistance
ISSN: 2211-3207
Titre abrégé: Int J Parasitol Drugs Drug Resist
Pays: Netherlands
ID NLM: 101576715

Informations de publication

Date de publication:
12 2021
Historique:
received: 20 04 2021
revised: 18 10 2021
accepted: 18 10 2021
pubmed: 10 11 2021
medline: 7 1 2022
entrez: 9 11 2021
Statut: ppublish

Résumé

P-glycoproteins from the ATP-binding cassette transporter family are responsible for drug evasion by bacterial pathogens and neoplastic cells. More recently, these multidrug resistance transporters have been investigated for contributions to drug resistance in nematode parasites. In this study, we cloned and characterized the P-glycoprotein Tca-Pgp-11.1 from Toxocara canis, the canine intestinal ascarid. Large numbers of Tca-Pgp-11 transcripts were observed in the intestine of adult male and female worms. Heterologous expression studies confirmed sensitivity to known P-glycoprotein inhibitors. Interestingly, the competitive inhibitor verapamil had lower IC

Identifiants

pubmed: 34753107
pii: S2211-3207(21)00051-8
doi: 10.1016/j.ijpddr.2021.10.002
pmc: PMC8586496
pii:
doi:

Substances chimiques

ATP Binding Cassette Transporter, Subfamily B 0
ATP Binding Cassette Transporter, Subfamily B, Member 1 0
Anthelmintics 0
Pharmaceutical Preparations 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

191-203

Subventions

Organisme : NIAID NIH HHS
ID : R21 AI144493
Pays : United States

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.

Auteurs

Jeba R J Jesudoss Chelladurai (JRJ)

Department of Veterinary Pathology, Iowa State University College of Veterinary Medicine, USA; Department of Diagnostic Medicine/ Pathobiology, Kansas State University College of Veterinary Medicine, USA.

Douglas E Jones (DE)

Department of Veterinary Pathology, Iowa State University College of Veterinary Medicine, USA.

Matthew T Brewer (MT)

Department of Veterinary Pathology, Iowa State University College of Veterinary Medicine, USA. Electronic address: brewermt@iastate.edu.

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Classifications MeSH