Critical media attributes in E-beam sterilization of corneal tissue.

Cornea Corneal Transplantation Electrons Gamma Rays Sterilization
Biomechanical properties Cornea Electron beam irradiation Polarity Sterilization

Journal

Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144

Informations de publication

Date de publication:
15 01 2022
Historique:
received: 29 07 2021
revised: 20 10 2021
accepted: 21 10 2021
pubmed: 11 11 2021
medline: 28 1 2022
entrez: 10 11 2021
Statut: ppublish

Résumé

When ionizing irradiation interacts with a media, it can form reactive species that can react with the constituents of the system, leading to eradication of bioburden and sterilization of the tissue. Understanding the media's properties such as polarity is important to control and direct those reactive species to perform desired reactions. Using ethanol as a polarity modifier of water, we herein generated a series of media with varying relative polarities for electron beam (E-beam) irradiation of cornea at 25 kGy and studied how the irradiation media's polarity impacts properties of the cornea. After irradiation of corneal tissues, mechanical (tensile strength and modulus, elongation at break, and compression modulus), chemical, optical, structural, degradation, and biological properties of the corneal tissues were evaluated. Our study showed that irradiation in lower relative polarity media improved structural properties of the tissues yet reduced optical transmission; higher relative polarity reduced structural and optical properties of the cornea; and intermediate relative polarity (ethanol concentrations = 20-30% (v/v)) improved the structural properties, without compromising optical characteristics. Regardless of media polarity, irradiation did not negatively impact the biocompatibility of the corneal tissue. Our data shows that the absorbed ethanol can be flushed from the irradiated cornea to levels that are nontoxic to corneal and retinal cells. These findings suggest that the relative polarity of the irradiation media can be tuned to generate sterilized tissues, including corneal grafts, with engineered properties that are required for specific biomedical applications. STATEMENT OF SIGNIFICANCE: Extending the shelf-life of corneal tissue can improve general accessibility of cornea grafts for transplantation. Irradiation of donor corneas with E-beam is an emerging technology to sterilize the corneal tissues and enable their long-term storage at room temperature. Despite recent applications in clinical medicine, little is known about the effect of irradiation and preservation media's characteristics, such as polarity on the properties of irradiated corneas. Here, we have showed that the polarity of the media can be a valuable tool to change and control the properties of the irradiated tissue for transplantation.

Identifiants

DOI: 10.1016/j.actbio.2021.10.033 PMID: 34755604 PMC: PMC8738149
pubmed: 34755604
pii: S1742-7061(21)00704-2
doi: 10.1016/j.actbio.2021.10.033
pmc: PMC8738149
mid: NIHMS1752012
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

218-227

Subventions

Organisme : NEI NIH HHS
ID : K99 EY030553
Pays : United States

Informations de copyright

Copyright © 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare no conflict of interest.

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Auteurs

Sina Sharifi (S)

Disruptive Technology Laboratory, Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA. Electronic address: Sina_Sharifi@meei.harvard.edu.

Hannah Sharifi (H)

Disruptive Technology Laboratory, Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.

Ali Akbari (A)

Solid Tumor Research Center, Research Institute for Cellular and Molecular Medicine, Urmia University of Medical Sciences, Urmia, Iran.

Fengyang Lei (F)

Disruptive Technology Laboratory, Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.

Claes H Dohlman (CH)

Disruptive Technology Laboratory, Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.

Miguel Gonzalez-Andrades (M)

Disruptive Technology Laboratory, Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA; Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Department of Ophthalmology, Reina Sofia University Hospital and University of Cordoba, Cordoba, Spain.

Curtis Guild (C)

Centaur Technologies, Vernon, CT, USA.

Eleftherios I Paschalis (EI)

Disruptive Technology Laboratory, Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.

James Chodosh (J)

Disruptive Technology Laboratory, Massachusetts Eye and Ear and Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA. Electronic address: James_Chodosh@meei.harvard.edu.

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Classifications MeSH

questionsmedicales.fr › Organes des sens › Oeil › Pôle antérieur du bulbe oculaire › Cornée › Critical media attributes in E-beam...
questionsmedicales.fr › Procédures de chirurgie opératoire › Transplantation › Transplantation de tissu › Transplantation de cornée › Critical media attributes in E-beam...
questionsmedicales.fr › Phénomènes physiques › Phénomènes magnétiques › Phénomènes électromagnétiques › Électrons › Critical media attributes in E-beam...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement ionisant › Rayons gamma › Critical media attributes in E-beam...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Contrôle des maladies transmissibles › Prévention des infections › Stérilisation › Critical media attributes in E-beam...