Study of optimal conditions for growth and osteogenic differentiation of dental pulp stem cells based on glucose and serum content.
DPSCs, dental pulp stem cells
Defined culture conditions
Dental pulp stem cells
Differentiation
HGHS, high glucose high serum
HGLS, high glucose low serum
LGHS, low glucose high serum
LGLS, low glucose low serum
Mesenchymal stem cells
Journal
Saudi journal of biological sciences
ISSN: 1319-562X
Titre abrégé: Saudi J Biol Sci
Pays: Saudi Arabia
ID NLM: 101543796
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
08
06
2021
revised:
18
06
2021
accepted:
30
06
2021
entrez:
11
11
2021
pubmed:
12
11
2021
medline:
12
11
2021
Statut:
ppublish
Résumé
Dental pulp stem cells (DPSCs) have shown promising characteristics in terms of their proliferation and osteogenic differentiation potential, which could be of greater benefit in regenerative dentistry. However, obstacles remain in the in vitro cultivation of DPSCs, which significantly affect their growth and differentiating ability. Therefore in this study, we demonstrated the growth and osteogenic differentiation of DPSCs in the presence of media containing different combinations of serum and glucose to get an optimized combination of both. DPSCs were cultured in media containing combinations of low glucose (LG), low serum (LS), high glucose (HG), and high serum (HS). The proliferation and osteogenic differentiation were assessed in DPSCs cultured with these different combinations of culture conditions. High glucose high serum condition significantly inhibited the proliferation of DPSCs and also affected their clonogenic potential, as evidenced by colony-forming units. Irrespective of the serum content, high glucose in the media also decreased the osteogenic potential of DPSCs confirmed by functional staining, and downregulation of osteogenesis-related genes. High glucose content in the culture media affects the growth and differentiation potential of the DPSCs. Hence, the culture conditions for the DPSCs should be reconsidered to utilize their maximum potential.
Identifiants
pubmed: 34759755
doi: 10.1016/j.sjbs.2021.06.101
pii: S1319-562X(21)00573-8
pmc: PMC8568704
doi:
Types de publication
Journal Article
Langues
eng
Pagination
6359-6364Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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