Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction.
Cell Biology
Cell Differentiation
Cell culture
Developmental biology
Flow Cytometry/Mass Cytometry
Molecular Biology
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 12 2021
17 12 2021
Historique:
entrez:
11
11
2021
pubmed:
12
11
2021
medline:
12
11
2021
Statut:
epublish
Résumé
Human trophoblasts arise from the morula as trophectoderm, which differentiates into cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast after implantation. Here, we present a robust step-by-step protocol to induce trophectoderm (TE) from naive human pluripotent stem cells (PSCs) corresponding to pre-implantation epiblast. Our culture system (TE induction and ACE condition) mimics the entire trophoblast development including the molecular events. For complete details on the use and execution of this protocol, please refer to Io et al. (2021).
Identifiants
pubmed: 34761233
doi: 10.1016/j.xpro.2021.100921
pii: S2666-1667(21)00627-4
pmc: PMC8567437
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100921Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
S.I. and Y.T. are co-inventors on a patent filing describing the generation of trophectoderm-like cells from naive human pluripotent stem cells.
Références
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