ATG7 immunohistochemical expression in malignant pleural mesothelioma. A preliminary report.
Journal
Histology and histopathology
ISSN: 1699-5848
Titre abrégé: Histol Histopathol
Pays: Spain
ID NLM: 8609357
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
pubmed:
12
11
2021
medline:
1
4
2022
entrez:
11
11
2021
Statut:
ppublish
Résumé
Literature evidence has demonstrated a high incidence of asbestos-related malignant pleural mesothelioma (MPM) in a Sicilian town (Biancavilla, Italy), where fluoro-edenite (FE) fibers were discovered some decades ago. As ATG7 immunohistochemical analysis has been ascribed as a prognostic tool of improved survival, we decided to investigate, in MPM patients, exposed and not exposed to FE fibers, the immunohistochemical expression of this autophagy-related protein named ATG7. We analyzed the correlation between ATG7 immunohistochemical level and clinicopathological parameters. Twenty MPM tissue samples, from patients with available clinical and follow-up data, were included in paraffin and processed for immunohistochemistry. The immunohistochemical results confirmed activation of the autophagic process in MPM. Densitometric and morphometric expressions of ATG7 were significantly increased in MPMs when compared to the control tissues. A significant association of a high level of ATG7 with increased survival was demonstrated, with a mean overall survival (OS) of 12.5 months for patients with high expression vs. a mean OS of 4.5 months for patients with low ATG7 expression. In addition, a significant correlation between ATG7 expression and the survival time of MPM patients was observed. This study represents a starting point to hypothesize the prognostic role of ATG7 which could be a reliable prognostic indicator in MPM.
Identifiants
pubmed: 34761371
pii: HH-18-396
doi: 10.14670/HH-18-396
doi:
Substances chimiques
Asbestos, Amphibole
0
Biomarkers, Tumor
0
fluor-edenite
0
ATG7 protein, human
EC 6.2.1.45
Autophagy-Related Protein 7
EC 6.2.1.45
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1301-1308Subventions
Organisme : 2020/2022 PIA.CE.RI., University of Catania, DIPREME project
ID : 20722142130
Références
Angelico G., Caltabiano R., Loreto C., Ieni A., Tuccari G., Ledda C. and Rapisarda V. (2018). Immunohistochemical expression of aquaporin-1 in fluoro-edenite-induced malignant mesothelioma: A preliminary report. Int. J. Mol. Sci. 19, 685.
pmcid: PMC5877546
pubmed: 29495596
Blanquart C., Jaurand M. and Jean D. (2020). The biology of malignant mesothelioma and the relevance of preclinical models. Front. Oncol. 10, 338.
pmcid: PMC7109283
pubmed: 32269966
Botti J., Djavaheri-Mergny M., Pilatte Y. and Codogno P. (2006). Autophagy signaling and the cogwheels of cancer. Autophagy 2, 67- 73.
pubmed: 16874041
Broggi G., Filetti V, Ieni A., Rapisarda V., Ledda C., Vitale E., Varricchio S., Russo D., Lombardo C., Tuccari G., Caltabiano R and Loreto C. (2020a). MacroH2A1 immunoexpression in breast cancer. Front. Oncol. 10, 1519.
pmcid: PMC7471871
pubmed: 32974186
Broggi G., Ieni A., Russo D., Varricchio S., Puzzo L., Russo A., Reibaldi M., Longo A., Tuccari G., Staibano S. and Caltabiano R. (2020b). The macro-autophagy-related protein Beclin-1 immunohistochemical expression correlates with tumor cell type and clinical behavior of uveal melanoma. Front. Oncol. 10, 589849.
pmcid: PMC7714947
pubmed: 33330070
Broggi G., Angelico G., Filetti V., Ledda C., Lombardo C., Vitale E., Rapisarda V., Loreto C. and Caltabiano R. (2021). Immunohistochemical expression of serine and arginine-rich splicing factor 1 (SRSF1) in fluoro-edenite-induced malignant mesothelioma: A preliminary study. Int. J. Environ. Res. Public Health 18, 6249.
pmcid: PMC8296067
pubmed: 34207798
Cianfanelli V., Fuoco C., Lorente M., Salazar M., Quondamatteo F., Gherardini P.F., De Zio D., Nazio F., Antonidi M., D’Orazio M., Skobo T., Bordi M., Rohde M., Dalla Valle L., Helmer-Citterich M., Gretzmeier C., Dengjel J., Fimia G.M., Piacentini M., Di Bartolomeo S., Velasco G. and Cecconi F . (2015). AMBRA1 links autophagy to cell proliferation and tumourigenesis by promoting c-Myc dephosphorylation and degradation. Nat. Cell Biol. 17, 20-30.
pmcid: PMC4976803
pubmed: 25438055
Cui Z., Zhang Y., Xia K., Yan Q., Kong H., Zhang J., Zuo X., Shi J., Wang L., Zhu Y. and Fan C. (2018). Nanodiamond autophagy inhibitor allosterically improves the arsenical-based therapy of solid tumors. Nat. Commun. 9, 4347.
pmcid: PMC6195623
pubmed: 30341298
Delgermaa V., Takahashi K., Park E.K., Le G.V., Hara T. and Sorahan T. (2011). Global mesothelioma deaths reported to the World Health Organization between 1994 and 2008. Bull. World Health Organ. 89, 7.
pmcid: PMC3209980
pubmed: 22084509
Ding S. and Hong Y. (2020). The fluorescence toolbox for visualizing autophagy. Chem. Soc. Rev. 49, 8354-8389.
pubmed: 33156302
Dyczynski M., Yu Y., Otrocka M., Parpal S., Braga T., Henley A.B., Zazzi H., Lerner M., Wennerberg K., Viklund J., Martinsson J., Grandér D., De Milito A. and Pokrovskaja Tamm K. (2018). Targeting autophagy by small molecule inhibitors of vacuolar protein sorting 34 (Vps34) improves the sensitivity of breast cancer cells to sunitinib. Cancer Lett. 435, 32-43.
pubmed: 30055290
Fazzo L., Minelli G., Bruno C., Comba P., Conti S., De Santis M., Zona A., Binazzi A., Magnani C., Marinaccio A. and Iavarone I. (2020). Early mortality from malignant mesothelioma in Italy as a proxy of environmental exposure to asbestos in children. Ann. Ist. Super. Sanità 56, 478-86.
pubmed: 33346174
Filetti V., Vitale E., Broggi G., Hagnäs M.P., Candido S., Spina A. and Lombardo C. (2020a). Update of in vitro, in vivo and ex vivo fluoro edenite effects on malignant mesothelioma: A systematic review (Review). Biomed. Rep. 13, 60.
pmcid: PMC7605121
pubmed: 33149905
Filetti V., Falzone L., Rapisarda V., Caltabiano R., Graziano A.C.E., Ledda C. and Loreto C. (2020b). Modulation of microRNA expression levels after naturally occurring asbestiform fibers exposure as a diagnostic biomarker of mesothelial neoplastic transformation. Ecotoxicol. Environ. Saf. 198, 110640.
pubmed: 32330788
Fimia G.M. and Piacentini M. (2010). Regulation of autophagy in mammals and its interplay with apoptosis. Cell Mol. Life Sci. 67, 1581-1588.
pubmed: 20165902
Follo C., Barbone D., Richards W.G., Bueno R. and Broaddus V.C. (2016). Autophagy initiation correlates with the autophagic flux in 3D models of mesothelioma and with patient outcome. Autophagy 12, 1180-1194.
pmcid: PMC4990992
pubmed: 27097020
Follo C., Cheng Y., Richards W.G., Bueno R. and Broaddus V.C. (2018). Inhibition of autophagy initiation potentiates chemosensitivity in mesothelioma. Mol. Carcinog. 57, 319-332.
pubmed: 29073722
Follo C., Barbone D., Richards W.G., Bueno R. and Courtney Broaddus V. (2019a). Autophagy in 3D in vitro and ex vivo cancer models. Methods Mol. Biol. 1880, 491-510.
pubmed: 30610717
Follo C., Cheng Y., Richards W.G., Bueno R. and Broaddus V.C. (2019b). Autophagy facilitates the release of immunogenic signals following chemotherapy in 3D models of mesothelioma. Mol. Carcinog. 58, 1754-1769.
pubmed: 31215708
Galateau-Salle F., Churg A., Roggli V. and Travis W.D. (2016). World Health Organization CoMMittee for Tumors of the Pleura. The 2015 World Health Organization Classification of Tumors of the Pleura: Advances since the 2004 Classification. J. Thorac. Oncol. 11, 142- 154.
pubmed: 26811225
Galluzzi L., Pietrocola F., Bravo-San Pedro J.M., Amaravadi R.K., Baehrecke E.H., Cecconi F., Codogno P., Debnath J., Gewirtz D.A., Karantza V., Kimmelman A., Kumar S., Levine B., Maiuri M.C., Martin S.J., Penninger J., Piacentini M., Rubinsztein D.C., Simon H.- U., Simonsen A., Thorburn A.M., Velasco G., Ryan K.M. and Kroemer G. (2015). Autophagy in malignant transformation and cancer progression. EMBO J. 34, 856-880.
pmcid: PMC4388596
pubmed: 25712477
Gugnoni M., Sancisi V., Manzotti G., Gandolfi G. and Ciarrocchi A. (2016). Autophagy and epithelial-mesenchymal transition: an intricate interplay in cancer. Cell Death Dis. 7, e2520.
pmcid: PMC5260980
pubmed: 27929542
Hill G.D., Mangum J.B., Moss O.R. and Everitt J.I. (2003). Soluble ICAM-1, MCP-1, and MIP-2 protein secretion by rat pleural mesothelial cells following exposure to amosite asbestos. Exp. Lung Res. 29, 277-290.
pubmed: 12746042
Klionsky D.J. (2005). Autophagy. Curr. Biol. 15, 282-283.
pubmed: 15854889
Kopecka J., Salaroglio I.C., Righi L., Libener R., Orecchia S., Grosso F., Milosevic V., Ananthanarayanan P., Ricci L., Capelletto E., Pradotto M., Napoli F., Di Maio M., Novello S., Rubinstein M., Scagliotti G.V. and Riganti C. (2018). Loss of C/EBP-β LIP drives cisplatin resistance in malignant pleural mesothelioma. Lung Cancer 120, 34- 45.
pubmed: 29748013
Ledda C., Loreto C., Pomara C., Rapisarda G., Fiore M., Ferrante M., Bracci M., Santarelli L., Fenga C. and Rapisarda V. (2016a). Sheep lymph-nodes as a biological indicator of environmental exposure to fluoro-edenite. Environ. Res. 147, 97-101.
pubmed: 26855127
Ledda C., Loreto C., Bracci M., Mangano D., Migliore M., Ricceri V., Musumeci A., Costa C., Pomara C. and Rapisarda V. (2016b). High risk of pleural plaques and parenchymal abnormalities in women living in Biancavilla (Italy). Future Oncol. 12, 63-65.
pubmed: 27624550
Lee I.H., Kawai Y., Fergusson M.M., Rovira I.I., Bishop A.J.R., Motoyama N., Cao L. and Finkel T. (2012). Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336, 225-228.
pmcid: PMC4721513
pubmed: 22499945
Lee Y.A., Noon L.A., Akat K.M., Ybanez M.D., Lee T.-F., Berres M.-L., Fujiwara N., Goossens N., Chou H.I., Parrin-Nejad F.P., Khambu B., Kramer E.G.M., Gordon R., Pfleger C., Germain D., John G.R., Campbell K.N., Yue Z., Yin X.M., Cuervo A.M., Czaja M.J., Fiel H.I., Hoshida Y. and Friedman S.L. (2018). Autophagy is a gatekeeper of hepatic differentiation and carcinogenesis by controlling the degradation of Yap. Nat. Commun. 9, 4962.
pmcid: PMC6251897
pubmed: 30470740
Leonardi R., Talic N.F. and Loreto C. (2007). MMP-13 (collagenase 3) immunolocalisation during initial orthodontic tooth movement in rats. Acta Histochem. 109, 215-220.
pubmed: 17350083
Levine B. and Klionsky D.J. (2004). Development by self-digestion: molecular mechanisms and biological functions of autophagy. Dev. Cell. 6, 463-477.
pubmed: 15068787
Levy J.M.M., Towers C.G. and Thorburn A. (2017). Targeting autophagy in cancer. Nat. Rev. Cancer 17, 528-542.
pmcid: PMC5975367
pubmed: 28751651
Li Y.N., Radner S., French M.M., Pinzón-Duarte G., Daly G.H., Burgeson R.E., Koch M. and Brunken W.J. (2012). The γ3 chain of laminin is widely but differentially expressed in murine basement membranes: Expression and functional studies. Matrix Biol. 31, 120- 134.
pmcid: PMC3288684
pubmed: 22222602
Loreto C., Rapisarda V., Carnazza M.L., Musumeci G., Valentino M., 1307 ATG7 immunohistochemical expression in malignant pleural mesothelioma Fenga C. and Martinez G. (2008). Fluoro-edenite fibres induce lung cell apoptosis: An in vivo study. Histol. Histopathol. 23, 319- 326.
pubmed: 18072089
Loreto C., Lombardo C., Caltabiano R., Filetti V., Vitale E., Seminara D., Castorina S., Fenga C., Ledda C. and Rapisarda V. (2019). Immunohistochemical expression and localization of MMP-9, MMP13, E-cadherin and Ki-67 in road pavers’ skin chronically exposed to bitumen products. Histol. Histopathol. 34, 1141-1150.
pubmed: 30973180
Loreto C., Lombardo C., Caltabiano R., Ledda C., Hagnäs M.P., Filetti V. and Rapisarda V. (2020a). An in vivo immunohistochemical study on macroh2a.1 in lung and lymph-node tissues exposed to an asbestiform fiber. Cur. Molec. Med. 20, 653-660.
pubmed: 32077824
Loreto C., Caltabiano R., Graziano A.C.E., Castorina S., Lombardo C., Filetti V., Vitale E., Rapisarda G., Cardile V., Ledda C. and Rapisarda V. (2020b). Defense and protection mechanisms in lung exposed to asbestiform fiber: The role of macrophage migration inhibitory factor and heme oxygenase-1. Europ. J. Histochem. 64, 3073.
pmcid: PMC7171426
pubmed: 32312030
Loreto C., Filetti V., Almeida L.E., La Rosa G.R.M., Leonardi R., Grippaudo C. and Lo Giudice A. (2020c). MMP-7 and MMP-9 are overexpressed in the synovial tissue from severe temporomandibular joint dysfunction. Eur. J. Histochem. 64, 3113.
pmcid: PMC7171425
pubmed: 32312031
Loreto C., Ledda C., Tumino R., Lombardo C., Vitale E., Filetti V., Caltabiano R. and Rapisarda V. (2020d). Activation of caspase-3 in malignant mesothelioma induced by asbestiform fiber: an in vivo study. J. Biol. Regul. Homeost. Agents. 34, 1163-1166.
pubmed: 32691574
Maes H., Rubio N., Garg D.A. and Agostinis P. (2013). Autophagy: shaping the tumour microenvironment and therapeutic response. Trends Mol. Med. 19, 428-446.
pubmed: 23714574
Maiuri M.C., Zalckvar E., Kimchi A. and Kroemer G. (2007). Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat. Rev. Mol. Cell Biol. 8, 741-752.
pubmed: 17717517
Marcq E., Siozopoulou V., de Waele J., van Audenaerde J., Zwaenepoel K., Santermans E., Hens N.,Pauwels P., van Meerbeeck J.P. and Smits E.L. (2016). Prognostic and predictive aspects of the tumor immune microenvironment and immune checkpoints in malignant pleural mesothelioma. Oncoimmunology 6, e1261241.
pmcid: PMC5283621
pubmed: 28197385
Mariño G., Niso-Santano M., Baehrecke E.H. and Kroemer G. (2014). Self-consuption: the interplay of autophagy and apoptosis. Nat. Rev. Mol. Cell Biol. 15, 81-94.
pmcid: PMC3970201
pubmed: 24401948
Marzullo A., Serio G., Pezzuto F., Fortarezza F., Cazzato G., Caporusso C., Lettini T., Cavone D., Delfino M.C. and Vimercati L. (2020). A single liver metastasis from pleural biphasic mesothelioma. Diagn. 10, 555.
pmcid: PMC7460021
pubmed: 32759747
Pasetto R., Zona A., Fazzo L., Binazzi A., Bruno C., Pirastu R., Comba P. and Marinaccio A. (2019). Proportion of mesothelioma attributable to living in industrially contaminated areas in Italy. Scand. J. Work. Environ. Health 45, 444-449.
pubmed: 30815702
Perut F., Sbrana F.V., Avnet S., De Milito A. and Baldini N. (2018). Spheroid-based 3D cell cultures identify salinomycin as a promising drug for the treatment of chondrosarcoma. J. Orthop. Res. 36, 2305- 2312.
pubmed: 29469166
Pezzuto F., Serio G., Fortarezza F., Scattone A., Caporusso C., Punzi A., Cavone D., Pennella A., Marzullo A. and Vimercati L. (2020). Prognostic value of Ki67 percentage, WT-1 expression and p16/CDKN2A deletion in diffuse malignant peritoneal mesothelioma: A single-centre cohort study. Diagnostics 10, 386.
pmcid: PMC7345555
pubmed: 32526924
Serio G., Vimercati L., Pennella A., Gentile M., Cavone D., Buonadonna A.L., Scattone A., Fortarezza F., De Palma A. and Marzullo A. (2018). Genomic changes of chromosomes 8p23.1 and 1q21: Novel mutations in malignant mesothelioma. Lung Cancer 126, 106- 111.
pubmed: 30527173
White E. (2015). The role for autophagy in cancer. J. Clin. Invest. 125, 42-46.
pmcid: PMC4382247
pubmed: 25654549
Xiong J. (2015). Atg7 in development and disease: panacea or Pandora’s Box? Protein Cell 6, 722-734.
pmcid: PMC4598325
pubmed: 26404030
Yonekawa T. and Thorburn A. (2013). Autophagy and cell death. Essays Biochem. 55, 105-117.
pmcid: PMC3894632
pubmed: 24070475
Zhang W. Q., Dai Y. Y., Hsu P. C., Wang H., Cheng L., Yang Y. L., Wang Y. C., Xu Z. D., Liu S., Chan G., Hu B., Li H., Jablons D. M. and You L. (2017). Targeting YAP in malignant pleural mesothelioma. J. Cell. Mol. Med. 21, 2663-2676.
pmcid: PMC5661117
pubmed: 28470935
Zhou R., Yazdi A.S., Menu P. and Tschopp J. (2011). A role for mitochondria in NLRP3 inflammasome activation. Nature 469, 221- 225.
pubmed: 21124315
Zona A., Fazzo L., Minelli G., De Santis M., Bruno C., Conti S. and Comba P. (2019). Peritoneal mesothelioma mortality in Italy: Spatial analysis and search for asbestos exposure sources. Cancer Epidemiol. 60, 162-167.
pubmed: 31030080