Expression of macrophage migration inhibitory factor and its receptor CD74 in systemic sclerosis.
CD74
MIF
immunohistochemistry
sclerosis systemic
skin
Journal
Central-European journal of immunology
ISSN: 1426-3912
Titre abrégé: Cent Eur J Immunol
Pays: Poland
ID NLM: 9702239
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
08
2020
accepted:
10
05
2021
entrez:
12
11
2021
pubmed:
13
11
2021
medline:
13
11
2021
Statut:
ppublish
Résumé
Macrophage migration inhibitory factor (MIF) has been associated with the pathogenesis of several rheumatic diseases. In systemic sclerosis (SSc) it has been shown that MIF expression is dysregulated in serum and skin. However, the MIF receptor, CD74, has been poorly investigated and its potential role in the pathogenesis of SSc remains unknown. This study aimed to analyze mRNA, tissue, and serum expression of MIF and CD74 in patients with limited (lcSSc) and diffuse (dcSSc) systemic sclerosis. A case-control study in 20 SSc patients and 20 control subjects (CS) from southern México was conducted. MIF and CD74 mRNA expression levels were quantified by real-time PCR, MIF serum levels were measured by an ELISA kit, and MIF and its receptor CD74 were evaluated by immunohistochemistry of skin biopsies. MIF mRNA expression was significantly higher in CS than in SSc patients (p = 0.02), while CD74 showed no differences between patients and CS. MIF serum levels were similar between SSc patients and CS: dcSSc = 3.82 ng/ml, lcSSc = 3.57 ng/ml, and CS = 3.28 ng/ml. In skin biopsies of SSc, MIF and CD74 were enhanced in keratinocytes, while they showed decreased expression in endothelial cells. On the other hand, the staining of CD74 was high in fibroblasts of dcSSc patients. Our findings show MIF and CD74 deregulation at the transcriptional and translational levels in SSc, which might be associated with the proinflammatory process leading to tissue remodeling and excessive fibrosis in SSc.
Identifiants
pubmed: 34764810
doi: 10.5114/ceji.2021.109756
pii: 45372
pmc: PMC8574103
doi:
Types de publication
Journal Article
Langues
eng
Pagination
375-383Informations de copyright
Copyright © 2021 Termedia.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Acta Biochim Biophys Sin (Shanghai). 2008 May;40(5):426-36
pubmed: 18465028
Science. 2015 Jan 23;347(6220):1260419
pubmed: 25613900
Immunity. 2006 Oct;25(4):595-606
pubmed: 17045821
Rheumatology (Oxford). 2017 Nov 1;56(11):1970-1981
pubmed: 28968684
Clin Exp Rheumatol. 2015 Jul-Aug;33(4 Suppl 91):S98-105
pubmed: 26005773
Front Immunol. 2015 Jun 08;6:272
pubmed: 26106387
Int Immunopharmacol. 2004 Feb;4(2):223-34
pubmed: 14996414
Arthritis Rheum. 2013 Nov;65(11):2737-47
pubmed: 24122180
J Immunol. 2009 Jun 1;182(11):6896-906
pubmed: 19454686
Clin Exp Immunol. 2008 May;152(2):328-35
pubmed: 18355352
Clin Rheumatol. 2008 Oct;27(10):1307-11
pubmed: 18618071
Clin Rheumatol. 2019 Jun;38(6):1643-1654
pubmed: 30747392
Am J Physiol. 1998 Apr;274(4):R1058-64
pubmed: 9575969
PLoS One. 2015 Mar 31;10(3):e0122482
pubmed: 25826375
Biochim Biophys Acta. 2000 Jan 3;1500(1):1-9
pubmed: 10564712
J Dent Res. 2012 Jul;91(7):651-8
pubmed: 22302144
Am J Pathol. 2005 Dec;167(6):1519-29
pubmed: 16314467
Indian J Dermatol. 2013 Mar;58(2):157
pubmed: 23716815
Immunol Cell Biol. 2003 Apr;81(2):137-43
pubmed: 12631237
Lancet. 2017 Oct 7;390(10103):1685-1699
pubmed: 28413064
Cell Signal. 2019 May;57:76-88
pubmed: 30682543
FEBS Lett. 2009 Sep 3;583(17):2749-57
pubmed: 19665027
Arthritis Rheum. 2006 Nov;54(11):3661-9
pubmed: 17075815
Open Rheumatol J. 2014 Sep 29;8:29-42
pubmed: 25328554
J Dermatol. 2018 Feb;45(2):128-138
pubmed: 29226387
Curr Opin Rheumatol. 2012 Mar;24(2):165-70
pubmed: 22269658
Nat Rev Immunol. 2003 Oct;3(10):791-800
pubmed: 14502271
J Dermatol Sci. 2005 Feb;37(2):65-73
pubmed: 15659324
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
J Rheumatol Suppl. 2011 Jan;86:3-8
pubmed: 21196592
Ann Rheum Dis. 2003 May;62(5):460-4
pubmed: 12695161
Cells. 2017 Feb 10;6(1):
pubmed: 28208600
J Biol Chem. 2008 Feb 1;283(5):2784-92
pubmed: 18056708
Clin Transl Immunology. 2018 Dec 06;7(12):e1042
pubmed: 30546906
Biotechnol Genet Eng Rev. 2013;29:49-60
pubmed: 24568252
Biochim Biophys Acta. 2016 Jun;1863(6 Pt A):1269-81
pubmed: 27033518
FASEB J. 2016 Nov;30(11):3870-3877
pubmed: 27528627
Anal Biochem. 1987 Apr;162(1):156-9
pubmed: 2440339