Coupling Between Production of Ribosomal RNA and Maturation: Just at the Beginning.
RNA folding
RNA polymerase I (Pol I)
premature termination of transcription
ribosomal RNA (rRNA) genes
ribosomal RNA (rRNA) processing
termination of transcription
transcription
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2021
2021
Historique:
received:
17
09
2021
accepted:
12
10
2021
entrez:
12
11
2021
pubmed:
13
11
2021
medline:
13
11
2021
Statut:
epublish
Résumé
Ribosomal RNA (rRNA) production represents the most active transcription in the cell. Synthesis of the large rRNA precursors (35S/47S in yeast/human) is achieved by up to hundreds of RNA polymerase I (Pol I) enzymes simultaneously transcribing a single rRNA gene. In this review, we present recent advances in understanding the coupling between rRNA production and nascent rRNA folding. Mapping of the distribution of Pol I along ribosomal DNA at nucleotide resolution, using either native elongating transcript sequencing (NET-Seq) or crosslinking and analysis of cDNAs (CRAC), revealed frequent Pol I pausing, and CRAC results revealed a direct coupling between pausing and nascent RNA folding. High density of Pol I per gene imposes topological constraints that establish a defined pattern of polymerase distribution along the gene, with a persistent spacing between transcribing enzymes. RNA folding during transcription directly acts as an anti-pausing mechanism, implying that proper folding of the nascent rRNA favors elongation
Identifiants
pubmed: 34765647
doi: 10.3389/fmolb.2021.778778
pii: 778778
pmc: PMC8575686
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
778778Informations de copyright
Copyright © 2021 Azouzi, Jaafar, Dez, Abou Merhi, Lesne, Henras and Gadal.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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