Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels.
CyTOF
Immunophenotyping
Mass cytometry
Maxpar
Metal-tagged antibodies
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
12
11
2021
pubmed:
13
11
2021
medline:
8
1
2022
Statut:
ppublish
Résumé
Mass cytometry, or cytometry by time-of-flight (the basis for Fluidigm
Identifiants
pubmed: 34766269
doi: 10.1007/978-1-0716-1771-7_9
doi:
Substances chimiques
Antibodies
0
Biomarkers
0
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-145Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Bagwell CB (2020) Multi-site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system. Cytometry B Clin Cytom 98(2):146–160. https://doi.org/10.1002/cyto.b.21858
doi: 10.1002/cyto.b.21858
pubmed: 31758746
Fluidigm Corporation (2020) Maxpar direct immune profiling assay cell staining and data acquisition user guide (400286)
Fluidigm Corporation (2020) Maxpar cytoplasmic/secreted antigen staining with fresh fix (400279)
Fluidigm Corporation (2019) Maxpar antibody labeling user guide (PRD002)
Fluidigm Corporation (2020) Helios, a CyTOF System User Guide (400250)
Fluidigm Corporation (2019) Technical note: approach to bivariate analysis of data acquired using the Maxpar direct immune profiling assay (400248)
Fluidigm Corporation (2020) Maxpar Panel Designer User Guide (100-9557).
Fluidigm Corporation (2020) Impact of cryopreservation on performance of the Maxpar direct immune profiling system (FLDM-00089).