Methotrexate Alters the Expression of microRNA in Fibroblast-like Synovial Cells in Rheumatoid Arthritis.
Arthritis, Rheumatoid
/ drug therapy
Cell Proliferation
/ drug effects
Cells, Cultured
Down-Regulation
/ drug effects
Fibroblasts
/ drug effects
Gene Expression Regulation
/ drug effects
Humans
Methotrexate
/ pharmacology
MicroRNAs
/ genetics
Synovial Membrane
/ drug effects
Synoviocytes
/ drug effects
Up-Regulation
/ drug effects
MTX
fibroblast-like synovial cells
miR-877-3p
microRNA
rheumatoid arthritis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
26 Oct 2021
26 Oct 2021
Historique:
received:
06
10
2021
revised:
21
10
2021
accepted:
22
10
2021
entrez:
13
11
2021
pubmed:
14
11
2021
medline:
16
12
2021
Statut:
epublish
Résumé
We aimed to investigate the effect of methotrexate (MTX) on microRNA modulation in rheumatoid arthritis fibroblast-like synovial cells (RA-FLS). RA-FLS were treated with MTX for 48 h. We then performed miRNA array analysis to investigate differentially expressed miRNAs. Transfection with miR-877-3p precursor and inhibitor were used to investigate the functional role of miR-877-3p in RA-FLS. Gene ontology analysis was used to investigate the cellular processes involving miR-877-3p. The production of cytokines/chemokines was screened by multiplex cytokine/chemokine bead assay and confirmed by ELISA and quantitative real-time PCR. The migratory and proliferative activities of RA-FLS were analyzed by wound healing assay and MKI-67 expression. MTX treatment altered the expression of 13 miRNAs (seven were upregulated and six were downregulated). Among them, quantitative real-time PCR confirmed that miR-877-3p was upregulated in response to MTX (1.79 ± 0.46-fold,
Identifiants
pubmed: 34768991
pii: ijms222111561
doi: 10.3390/ijms222111561
pmc: PMC8584010
pii:
doi:
Substances chimiques
MicroRNAs
0
Methotrexate
YL5FZ2Y5U1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : Grants-in-Aid for Scientific Research 16K19605 to Dr. Iwamoto
Références
Oncotarget. 2016 Aug 9;7(32):51773-51783
pubmed: 27429046
Arch Pharm Res. 2014 Oct;37(10):1286-94
pubmed: 24988987
Cancer Biol Med. 2021 Jun 10;:
pubmed: 34110722
PLoS One. 2016 Mar 31;11(3):e0151246
pubmed: 27031951
Blood. 1980 Dec;56(6):947-58
pubmed: 7002232
EMBO Mol Med. 2012 Sep;4(9):896-909
pubmed: 22887866
Eur Rev Med Pharmacol Sci. 2018 Oct;22(20):6625-6632
pubmed: 30402834
Arthritis Rheum. 1988 Mar;31(3):315-24
pubmed: 3358796
Blood. 1989 Dec;74(8):2769-70
pubmed: 2510837
Ther Adv Musculoskelet Dis. 2018 Feb;10(2):29-38
pubmed: 29387176
Br J Pharmacol. 2003 Feb;138(3):501-11
pubmed: 12569075
Clin Exp Immunol. 2004 Aug;137(2):430-6
pubmed: 15270863
Pharmacol Res. 2021 Nov;173:105887
pubmed: 34536550
Rheumatology (Oxford). 2015 Jan;54(1):178-87
pubmed: 25118313
J Rheumatol. 2018 Jan;45(1):53-61
pubmed: 29142030
Front Pharmacol. 2021 Aug 26;12:716081
pubmed: 34512345
BMC Cancer. 2020 Sep 25;20(1):924
pubmed: 32977766
Curr Pharm Biotechnol. 2018;19(1):79-86
pubmed: 29667551
J Rheumatol. 2015 Jun;42(6):943-7
pubmed: 25934826
J Immunol. 2003 May 15;170(10):4986-95
pubmed: 12734342
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
J Rheumatol. 1987 Feb;14(1):9-11
pubmed: 3033240
Apoptosis. 2005 Aug;10(4):895-907
pubmed: 16133879
Ann Rheum Dis. 2001 Aug;60(8):729-35
pubmed: 11454634
Immunopharmacology. 2000 Apr;47(1):35-44
pubmed: 10708808
Cytokine. 2015 Dec;76(2):527-536
pubmed: 26321413
J Cell Physiol. 2017 Jul;232(7):1907-1913
pubmed: 27982429
J Cell Physiol. 2011 Jul;226(7):1868-78
pubmed: 21506117
Apoptosis. 2006 Mar;11(3):389-99
pubmed: 16520895
Z Rheumatol. 1992 May-Jun;51(3):121-6
pubmed: 1502858
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2746-51
pubmed: 16477010
Arthritis Res Ther. 2017 May 31;19(1):110
pubmed: 28569176
Arthritis Rheum. 1989 Sep;32(9):1065-73
pubmed: 2476134
Biochem Pharmacol. 2018 Sep;155:110-123
pubmed: 29944870
Ann Rheum Dis. 1996 Apr;55(4):237-42
pubmed: 8733440
Immunol Med. 2019 Dec;42(4):156-161
pubmed: 31770498
Cell. 2010 Oct 1;143(1):21-6
pubmed: 20887887
Clin Rheumatol. 2019 Mar;38(3):877-884
pubmed: 30511295
Curr Rheumatol Rep. 2020 May 13;22(6):17
pubmed: 32405712
Ann Rheum Dis. 2020 Jun;79(6):685-699
pubmed: 31969328
Front Immunol. 2021 Mar 03;12:633201
pubmed: 33746971
Ann Rheum Dis. 2010 Sep;69(9):1580-8
pubmed: 20699241
Glia. 2006 Jan 15;53(2):115-23
pubmed: 16206159
Exp Ther Med. 2019 Jul;18(1):312-319
pubmed: 31258667