Method Development and Validation for Omega-3 Fatty Acids (DHA and EPA) in Fish Using Gas Chromatography with Flame Ionization Detection (GC-FID).
gas chromatography
omega-3 fatty acid
validation methods
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
30 Oct 2021
30 Oct 2021
Historique:
received:
30
08
2021
revised:
19
10
2021
accepted:
26
10
2021
entrez:
13
11
2021
pubmed:
14
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Gas chromatography with flame ionization detection (GC-FID) has often been used to quantify fatty acids in fish. This study validated the common method for determining omega-3 fatty acids (DHA and EPA) in the raw and cooked warm-water fish, selayang, using GC-FID for subsequent evaluation on EPA and DHA retention using the Weibull model. The EPA and DHA were separated using a high-polarity capillary GC HP-88 column (60 m length, 0.25 mm ID, 0.2 μm DF) with a total run time of 45.87 min. The method was validated in linearity, precision, accuracy, specificity and sensitivity based on ICH requirements. In addition, it was found that the method had a high recovery rate (>95%) and good precision (RSD ≤ 2%) with overall RSDs ranging below 0.001% for both omega-3 PUFA. In conclusion, this method identified and quantified fatty acids and omega-3 accurately and precisely and can be used effectively for routine FAME analysis in fish samples.
Identifiants
pubmed: 34771001
pii: molecules26216592
doi: 10.3390/molecules26216592
pmc: PMC8588356
pii:
doi:
Substances chimiques
Fatty Acids, Omega-3
0
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Universiti Putra Malaysia
ID : GP-IPS/2021/9701900
Références
Biochem Pharmacol. 2009 Mar 15;77(6):937-46
pubmed: 19022225
Can J Biochem Physiol. 1959 Aug;37(8):911-7
pubmed: 13671378
J Chromatogr A. 2016 Jul 22;1456:235-41
pubmed: 27320376
Ann Gastroenterol. 2016 Jan-Mar;29(1):37-43
pubmed: 26752948
Forensic Sci Int. 2007 Jan 17;165(2-3):216-24
pubmed: 16781833
Br J Nutr. 2012 Jun;107 Suppl 2:S201-13
pubmed: 22591894
JAMA. 2016 Feb 2;315(5):528
pubmed: 26836746
ACS Omega. 2021 Jan 04;6(2):1129-1137
pubmed: 33490772
Proc Nutr Soc. 2013 Nov;72(4):407-11
pubmed: 23953031
Chemosphere. 2017 Apr;173:116-123
pubmed: 28107709
Food Nutr Res. 2016 Aug 16;60:32697
pubmed: 27534846
Br J Nutr. 2012 Jun;107 Suppl 2:S8-22
pubmed: 22591906
Biomed Res Int. 2013;2013:284329
pubmed: 23509703
Food Chem. 2014 Dec 1;164:544-50
pubmed: 24996368
J Chromatogr Sci. 2013 Aug;51(7):599-607
pubmed: 23377649
J AOAC Int. 2009 Sep-Oct;92(5):1310-26
pubmed: 19916368
Mil Med. 2014 Nov;179(11 Suppl):95-105
pubmed: 25373092