Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
12 11 2021
12 11 2021
Historique:
received:
27
04
2021
accepted:
19
10
2021
entrez:
13
11
2021
pubmed:
14
11
2021
medline:
21
12
2021
Statut:
epublish
Résumé
Ammonia and its amine-containing derivatives are widely found in natural decomposition byproducts. Here, we conducted biased chemoreceptor screening to investigate the mechanisms by which different concentrations of ammonium salt, urea, and putrescine in rotten fruits affect feeding and oviposition behavior. We identified three ionotropic receptors, including the two broadly required IR25a and IR76b receptors, as well as the narrowly tuned IR51b receptor. These three IRs were fundamental in eliciting avoidance against nitrogenous waste products, which is mediated by bitter-sensing gustatory receptor neurons (GRNs). The aversion of nitrogenous wastes was evaluated by the cellular requirement by expressing Kir2.1 and behavioral recoveries of the mutants in bitter-sensing GRNs. Furthermore, by conducting electrophysiology assays, we confirmed that ammonia compounds are aversive in taste as they directly activated bitter-sensing GRNs. Therefore, our findings provide insights into the ecological roles of IRs as a means to detect and avoid toxic nitrogenous waste products in nature.
Identifiants
pubmed: 34773080
doi: 10.1038/s42003-021-02799-3
pii: 10.1038/s42003-021-02799-3
pmc: PMC8589963
doi:
Substances chimiques
Drosophila Proteins
0
IR76b protein, Drosophila
0
Ir52a protein, Drosophila
0
Ligand-Gated Ion Channels
0
Receptors, Ionotropic Glutamate
0
Sodium Channels
0
ionotropic receptor 25a, Drosophila
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1281Informations de copyright
© 2021. The Author(s).
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