DNA damage and repair in peripheral blood mononuclear cells after internal ex vivo irradiation of patient blood with
53BP1
DNA damage repair
Radioiodine therapy
γ-h2ax
Journal
European journal of nuclear medicine and molecular imaging
ISSN: 1619-7089
Titre abrégé: Eur J Nucl Med Mol Imaging
Pays: Germany
ID NLM: 101140988
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
21
07
2021
accepted:
26
10
2021
pubmed:
14
11
2021
medline:
12
4
2022
entrez:
13
11
2021
Statut:
ppublish
Résumé
The aim of this study was to provide a systematic approach to characterize DNA damage induction and repair in isolated peripheral blood mononuclear cells (PBMCs) after internal ex vivo irradiation with [ Blood of 33 patients of two centres was collected immediately before radioiodine therapy of differentiated thyroid cancer (DTC) and split into two samples. One sample served as non-irradiated control. The second sample was exposed to ionizing radiation by adding 1 ml of [ Thirty-two of 33 blood samples could be analysed. The mean absorbed dose to the blood in all irradiated samples was 50.1 ± 2.3 mGy. For all time points (0 h, 4 h, 24 h), the average number of γ-H2AX + 53BP1 foci per cell was significantly different when compared to baseline and the other time points. The average number of radiation-induced foci (RIF) per cell after irradiation was 0.72 ± 0.16 at t = 0 h, 0.26 ± 0.09 at t = 4 h and 0.04 ± 0.09 at t = 24 h. A monoexponential fit of the mean values of the three time points provided a decay rate of 0.25 ± 0.05 h This study provides novel data about the ex vivo DSB repair in internally irradiated PBMCs of patients before radionuclide therapy. Our findings show, in a large patient sample, that efficient repair occurs after internal irradiation with 50 mGy absorbed dose, and that the induction and repair rate after
Identifiants
pubmed: 34773472
doi: 10.1007/s00259-021-05605-8
pii: 10.1007/s00259-021-05605-8
pmc: PMC8940852
doi:
Substances chimiques
Histones
0
Iodine Radioisotopes
0
Iodine-131
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1447-1455Subventions
Organisme : h2020 euratom
ID : 755523
Informations de copyright
© 2021. The Author(s).
Références
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