Bifidobacterial β-Galactosidase-Mediated Production of Galacto-Oligosaccharides: Structural and Preliminary Functional Assessments.
Bifidobacterium
bifidogenic
gut microbiota
infant
microbiome
oligosaccharides
prebiotics
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2021
2021
Historique:
received:
30
07
2021
accepted:
27
09
2021
entrez:
15
11
2021
pubmed:
16
11
2021
medline:
16
11
2021
Statut:
epublish
Résumé
In the current study the ability of four previously characterized bifidobacterial β-galactosidases (designated here as BgaA, BgaC, BgaD, and BgaE) to produce galacto-oligosaccharides (GOS) was optimized. Of these enzymes, BgaA and BgaE were found to be promising candidates for GOS production (and the corresponding GOS mixtures were called GOS-A and GOS-E, respectively) with a GOS concentration of 19.0 and 40.3% (of the initial lactose), respectively. GOS-A and GOS-E were partially purified and structurally characterized. NMR analysis revealed that the predominant (non-lactose) disaccharide was allo-lactose in both purified GOS preparations. The predominant trisaccharide in GOS-A and GOS-E was shown to be 3'-galactosyllactose, with lower levels of 6'-galactosyllactose and 4'-galactosyllactose. These three oligosaccharides have also been reported to occur in human milk. Purified GOS-A and GOS-E were shown to be able to support bifidobacterial growth similar to a commercially available GOS. In addition, GOS-E and the commercially available GOS were shown to be capable of reducing
Identifiants
pubmed: 34777303
doi: 10.3389/fmicb.2021.750635
pmc: PMC8581567
doi:
Types de publication
Journal Article
Langues
eng
Pagination
750635Informations de copyright
Copyright © 2021 Ambrogi, Bottacini, Mac Sharry, van Breen, O’Keeffe, Walsh, Schoemaker, Cao, Kuipers, Lindner, Jimeno, Doyagüez, Hernandez-Hernandez, Moreno, Schoterman and van Sinderen.
Déclaration de conflit d'intérêts
MS, BK, LC, BS, and LC are employees of FrieslandCampina. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Microbiome. 2015 Apr 10;3:13
pubmed: 25922665
J Agric Food Chem. 2013 Mar 20;61(11):2748-54
pubmed: 23428168
Eur J Nutr. 2017 Aug;56(5):1919-1930
pubmed: 27295033
Microbiol Mol Biol Rev. 2017 Nov 8;81(4):
pubmed: 29118049
Benef Microbes. 2017 Aug 24;8(4):563-567
pubmed: 28726512
Front Microbiol. 2021 May 03;12:662959
pubmed: 34012427
Cell Host Microbe. 2019 Feb 13;25(2):324-335.e4
pubmed: 30763539
J Agric Food Chem. 2017 May 24;65(20):4184-4192
pubmed: 28466641
AMB Express. 2019 Jan 19;9(1):9
pubmed: 30661116
Annu Rev Nutr. 2000;20:699-722
pubmed: 10940350
FEMS Immunol Med Microbiol. 2007 Aug;50(3):380-8
pubmed: 17537177
Front Microbiol. 2016 Jun 28;7:979
pubmed: 27446020
Compr Rev Food Sci Food Saf. 2010 Sep;9(5):438-454
pubmed: 33467830
Nutr Rev. 2015 Jul;73(7):426-37
pubmed: 26081453
J Infect Dis. 1973 Jul;128(1):69-75
pubmed: 4577975
J Agric Food Chem. 2013 Feb 6;61(5):1081-7
pubmed: 23330921
Carbohydr Res. 2016 Apr 29;425:48-58
pubmed: 27035911
J Microbiol Biotechnol. 2014 May;24(5):675-82
pubmed: 24608564
PLoS One. 2014 Aug 04;9(8):e104056
pubmed: 25089712
World J Gastroenterol. 2008 Apr 7;14(13):2029-36
pubmed: 18395902
Appl Environ Microbiol. 2000 Apr;66(4):1379-84
pubmed: 10742215
Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):2108-13
pubmed: 22308390
Curr Microbiol. 2011 May;62(5):1448-54
pubmed: 21293857
J Microbiol Biotechnol. 2017 Aug 28;27(8):1392-1400
pubmed: 28535609
J Agric Food Chem. 2009 Sep 23;57(18):8488-95
pubmed: 19702273
BMC Genomics. 2018 Jan 8;19(1):33
pubmed: 29310579
World J Microbiol Biotechnol. 2016 Dec;32(12):197
pubmed: 27757792
Sci Rep. 2016 Dec 08;6:38560
pubmed: 27929046
Front Pediatr. 2019 Feb 27;7:47
pubmed: 30873394
Eur J Nutr. 2015 Feb;54(1):89-99
pubmed: 24671237
J Appl Microbiol. 2013 Apr;114(4):1132-46
pubmed: 23240984
Enzyme Microb Technol. 2012 Mar 10;50(3):188-94
pubmed: 22305174
World J Gastroenterol. 2006 Sep 14;12(34):5447-57
pubmed: 17006980
Annu Rev Food Sci Technol. 2019 Mar 25;10:75-102
pubmed: 30908947
Appl Environ Microbiol. 2006 Aug;72(8):5204-10
pubmed: 16885266
J Med Microbiol. 2009 Jan;58(Pt 1):37-48
pubmed: 19074651
Foodborne Pathog Dis. 2015 Apr;12(4):360-5
pubmed: 25692734
Int J Food Microbiol. 2015 Jun 16;203:109-21
pubmed: 25817019
Carbohydr Res. 2014 Dec 5;400:59-73
pubmed: 25444767
3 Biotech. 2017 May;7(1):79
pubmed: 28500401
Curr Issues Intest Microbiol. 2003 Sep;4(2):71-5
pubmed: 14503691
Springerplus. 2013 Aug 27;2:402
pubmed: 24024090
Infect Immun. 2006 Dec;74(12):6920-8
pubmed: 16982832
J Agric Food Chem. 2011 Apr 13;59(7):3366-72
pubmed: 21375323
Cell. 2006 Feb 24;124(4):715-27
pubmed: 16497583
J Appl Microbiol. 2014 Mar;116(3):477-91
pubmed: 24314205
J Nutr. 2016 Feb;146(2):358-67
pubmed: 26701795
Nutrients. 2021 Feb 22;13(2):
pubmed: 33671493
Br J Nutr. 2005 Nov;94(5):783-90
pubmed: 16277782
Int J Food Microbiol. 2011 Sep 1;149(1):81-7
pubmed: 21700354
Mol Microbiol. 2014 Oct 7;:
pubmed: 25287704
Front Immunol. 2019 Feb 12;10:94
pubmed: 30809221
Food Funct. 2020 Apr 30;11(4):3506-3515
pubmed: 32253406
J Pediatr Gastroenterol Nutr. 2009 Jan;48(1):82-8
pubmed: 19172129
Microbes Infect. 2015 Mar;17(3):173-83
pubmed: 25637951
J Chromatogr A. 2011 Oct 21;1218(42):7691-6
pubmed: 21641605
PLoS One. 2012;7(5):e36957
pubmed: 22606315
J Bacteriol. 2011 Sep;193(17):4540
pubmed: 21705601
J Agric Food Chem. 2006 Jul 12;54(14):4999-5006
pubmed: 16819908
Food Chem. 2013 Jan 15;136(2):689-94
pubmed: 23122115
Appl Environ Microbiol. 2001 Jun;67(6):2526-30
pubmed: 11375159
Pediatrics. 2006 Aug;118(2):511-21
pubmed: 16882802
Genes Nutr. 2011 Aug;6(3):285-306
pubmed: 21484167
J Pediatr. 1998 Jul;133(1):95-8
pubmed: 9672518
J Microbiol Methods. 2013 Sep;94(3):274-9
pubmed: 23835135
Sci Rep. 2019 Jan 30;9(1):960
pubmed: 30700794
World J Gastroenterol. 2008 Nov 14;14(42):6564-8
pubmed: 19030213