Internal Promoters and Their Effects on the Transcription of Operon Genes for Epothilone Production in
CRISPR-dCas9 activation
Myxococcus xanthus
biosynthetic gene cluster
epothilone
internal promoters
operon
transcription
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2021
2021
Historique:
received:
14
08
2021
accepted:
20
09
2021
entrez:
15
11
2021
pubmed:
16
11
2021
medline:
16
11
2021
Statut:
epublish
Résumé
The biosynthetic genes for secondary metabolites are often clustered into giant operons with no transcription terminator before the end. The long transcripts are frangible and the transcription efficiency declines along with the process. Internal promoters might occur in operons to coordinate the transcription of individual genes, but their effects on the transcription of operon genes and the yield of metabolites have been less investigated. Epothilones are a kind of antitumor polyketides synthesized by seven multifunctional enzymes encoded by a 56-kb operon. In this study, we identified multiple internal promoters in the epothilone operon. We performed CRISPR-dCas9-mediated transcription activation of internal promoters, combined activation of different promoters, and activation in different epothilone-producing
Identifiants
pubmed: 34778232
doi: 10.3389/fbioe.2021.758561
pii: 758561
pmc: PMC8579030
doi:
Types de publication
Journal Article
Langues
eng
Pagination
758561Informations de copyright
Copyright © 2021 Wang, Yue, Yuan, Hong, Hu and Li.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
BMC Microbiol. 2009 Dec 01;9:247
pubmed: 19951434
Mol Cell. 2004 Jun 4;14(5):647-56
pubmed: 15175159
Metab Eng. 2021 Jul;66:283-295
pubmed: 33930546
J Mol Biol. 2005 Feb 18;346(2):399-409
pubmed: 15670592
PLoS One. 2014 Mar 04;9(3):e90603
pubmed: 24594763
Intervirology. 2019;62(3-4):156-163
pubmed: 31430761
Nature. 2010 Mar 11;464(7286):250-5
pubmed: 20164839
Trends Genet. 2005 Jun;21(6):339-45
pubmed: 15922833
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D75-7
pubmed: 14681362
Nature. 2015 Jan 29;517(7536):583-8
pubmed: 25494202
Yi Chuan. 2015 Nov;37(11):1125-36
pubmed: 26582526
Science. 2009 Nov 27;326(5957):1268-71
pubmed: 19965477
Nucleic Acids Res. 2013 Aug;41(15):7429-37
pubmed: 23761437
PLoS One. 2013;8(2):e56321
pubmed: 23409173
PLoS One. 2017 Feb 3;12(2):e0171410
pubmed: 28158264
Gene. 2000 May 16;249(1-2):153-60
pubmed: 10831849
Sci Rep. 2013;3:2101
pubmed: 23812535
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8796-801
pubmed: 12077310
J Bacteriol. 2014 Feb;196(3):604-13
pubmed: 24244008
Appl Microbiol Biotechnol. 2018 Jul;102(13):5599-5610
pubmed: 29705958
Appl Environ Microbiol. 2002 Feb;68(2):449-55
pubmed: 11823177
Appl Environ Microbiol. 2008 Apr;74(7):1980-7
pubmed: 18245244
Cell Mol Life Sci. 2009 Dec;66(23):3755-75
pubmed: 19662496
J Bacteriol. 2013 Mar;195(6):1285-93
pubmed: 23316045
J Antibiot (Tokyo). 1996 Jun;49(6):560-3
pubmed: 8698639
Mol Microbiol. 1997 Feb;23(3):473-82
pubmed: 9044281
Genome Res. 2007 Oct;17(10):1478-85
pubmed: 17712020
Cancer Res. 1995 Jun 1;55(11):2325-33
pubmed: 7757983
J Bacteriol. 2001 Apr;183(8):2605-13
pubmed: 11274121
J Bacteriol. 2020 Apr 27;202(10):
pubmed: 32152218
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9139-44
pubmed: 12093906
Cell Rep. 2014 Dec 11;9(5):1966-1980
pubmed: 25544350
J Bacteriol. 1991 Jan;173(1):28-36
pubmed: 1702782
Mol Gen Genet. 1981;184(3):548-50
pubmed: 6278264
Transcription. 2011 Jan-Feb;2(1):9-14
pubmed: 21326903
Mol Med Rep. 2016 Aug;14(2):1227-34
pubmed: 27278638
J Antibiot (Tokyo). 2001 Feb;54(2):144-8
pubmed: 11302486
BMC Biotechnol. 2019 Jun 17;19(1):35
pubmed: 31208395
Sci Rep. 2020 Sep 3;10(1):14605
pubmed: 32884066
Nature. 2004 Jun 3;429(6991):571-4
pubmed: 15175754
Appl Environ Microbiol. 2000 Aug;66(8):3387-92
pubmed: 10919796
Chem Biol. 2000 Feb;7(2):97-109
pubmed: 10662695
FASEB J. 2014 Jan;28(1):143-52
pubmed: 24022402
Microb Cell Fact. 2017 May 23;16(1):92
pubmed: 28535774
Appl Microbiol Biotechnol. 2013 Aug;97(15):6857-66
pubmed: 23549746
PLoS Comput Biol. 2005 Aug;1(3):e25
pubmed: 16110342
Mol Microbiol. 1992 Sep;6(17):2419-22
pubmed: 1328815
Microb Cell Fact. 2018 Jan 29;17(1):15
pubmed: 29378572
J Bacteriol. 2007 May;189(10):3729-37
pubmed: 17369301
Microb Cell Fact. 2015 Jul 22;14:105
pubmed: 26194479
Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7317-21
pubmed: 8394017
Biochimie. 2015 Oct;117:129-37
pubmed: 25754521