Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scaling.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
03 01 2022
03 01 2022
Historique:
received:
28
06
2021
revised:
20
10
2021
accepted:
21
10
2021
entrez:
17
11
2021
pubmed:
18
11
2021
medline:
24
12
2021
Statut:
ppublish
Résumé
The function of cellular structures at the mesoscale is dependent on their geometry and proportionality to cell size. The mitotic spindle is a good example why length and shape of intracellular organelles matter. Spindle length determines the distance over which chromosomes will segregate, and spindle shape ensures bipolarity. While we still lack a systematic and quantitative understanding of subcellular morphology, new imaging techniques and volumetric data analysis promise novel insights into scaling relations across different species. Here, we introduce Spindle3D, an open-source plug-in that allows for the quantitative, consistent, and automated analysis of 3D fluorescent data of spindles and chromatin. We systematically analyze different mammalian cell types, including somatic cells, stem cells, and one- and two-cell embryos, to derive volumetric relations of spindle, chromatin, and the cell. Taken together, our data indicate that mitotic spindle width is a robust indicator of spindle volume, which correlates linearly with chromatin and cell volume both within single cell types and across mammalian species.
Identifiants
pubmed: 34787651
pii: 212842
doi: 10.1083/jcb.202106170
pmc: PMC8719715
pii:
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021 Kletter et al.
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