Multiwindow SRS Imaging Using a Rapid Widely Tunable Fiber Laser.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
30 11 2021
30 11 2021
Historique:
pubmed:
18
11
2021
medline:
15
12
2021
entrez:
17
11
2021
Statut:
ppublish
Résumé
Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally sensitive solid-state optical parametric oscillator (OPO) in a current SRS microscope. Moreover, chemically informative multiwindow SRS imaging across C-H, C-D, and fingerprint Raman regions is challenging due to the slow wavelength tuning speed of the solid-state OPO. In this work, we present a multiwindow SRS imaging system based on a compact and robust fiber laser with rapid and wide tuning capability. To address the relative intensity noise intrinsic to a fiber laser, we implemented autobalanced detection, which enhances the signal-to-noise ratio of stimulated Raman loss imaging by 23 times. We demonstrate high-quality SRS metabolic imaging of fungi, cancer cells, and
Identifiants
pubmed: 34787995
doi: 10.1021/acs.analchem.1c03604
pmc: PMC9713687
mid: NIHMS1848978
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
15703-15711Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM136223
Pays : United States
Références
Nat Biomed Eng. 2019 May;3(5):402-413
pubmed: 31036888
Opt Express. 2012 Jun 18;20(13):13958-65
pubmed: 22714461
Light Sci Appl. 2020 Feb 24;9:25
pubmed: 32133128
Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):E194-203
pubmed: 25550518
Nat Commun. 2015 Apr 17;6:6784
pubmed: 25881792
Sci Rep. 2017 Sep 6;7(1):10745
pubmed: 28878228
Science. 2008 Dec 19;322(5909):1857-61
pubmed: 19095943
Nat Commun. 2021 May 24;12(1):3052
pubmed: 34031374
Sci Adv. 2018 Nov 16;4(11):eaat7715
pubmed: 30456301
J Biophotonics. 2019 Sep;12(9):e201900028
pubmed: 31081280
Anal Chem. 2013 Jan 2;85(1):98-106
pubmed: 23198914
Science. 2015 Nov 27;350(6264):aaa8870
pubmed: 26612955
Theranostics. 2020 Apr 27;10(13):5865-5878
pubmed: 32483424
Biomed Opt Express. 2018 Nov 08;9(12):6116-6131
pubmed: 31065417
Opt Lett. 2021 Aug 1;46(15):3544-3547
pubmed: 34329220
J Phys Chem B. 2020 Apr 2;124(13):2591-2599
pubmed: 32176510
Acta Histochem Cytochem. 2018 Jun 26;51(3):101-110
pubmed: 30083018
Biomed Opt Express. 2019 Aug 05;10(9):4437-4449
pubmed: 31565500
Opt Express. 2013 Jun 3;21(11):13864-74
pubmed: 23736639
Adv Sci (Weinh). 2020 Aug 16;7(19):2001452
pubmed: 33042757
PLoS One. 2012;7(3):e33962
pubmed: 22479487
Nat Photonics. 2014 Feb 1;8(2):153-159
pubmed: 25313312
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 5;188:135-140
pubmed: 28709138
Anal Chem. 2020 Oct 6;92(19):13182-13191
pubmed: 32907318
Nat Methods. 2019 Sep;16(9):830-842
pubmed: 31471618
iScience. 2020 Mar 27;23(3):100953
pubmed: 32179477
Opt Lett. 2010 Jan 15;35(2):226-8
pubmed: 20081976
Opt Express. 2010 Nov 8;18(23):24019-24
pubmed: 21164749
Opt Express. 2010 Jun 21;18(13):13708-19
pubmed: 20588505
Nat Photonics. 2014;8:627-634
pubmed: 25621002
Nat Microbiol. 2016 Aug 01;1(10):16124
pubmed: 27670110
Light Sci Appl. 2018 May 04;7:17179
pubmed: 30839525
Biomed Opt Express. 2021 Sep 13;12(10):6228-6236
pubmed: 34745731