Red blood cell blood group A antigen level affects the ability of heparin and PfEMP1 antibodies to disrupt Plasmodium falciparum rosettes.
Histo-blood group ABO system
Malaria
Plasmodium falciparum
Rosetting
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
18 Nov 2021
18 Nov 2021
Historique:
received:
28
08
2021
accepted:
07
11
2021
entrez:
19
11
2021
pubmed:
20
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The histo-blood group ABO system has been associated with adverse outcomes in COVID-19, thromboembolic diseases and Plasmodium falciparum malaria. An integral part of the severe malaria pathogenesis is rosetting, the adherence of parasite infected red blood cells (RBCs) to uninfected RBCs. Rosetting is influenced by the host's ABO blood group (Bg) and rosettes formed in BgA have previously been shown to be more resilient to disruption by heparin and shield the parasite derived surface antigens from antibodies. However, data on rosetting in weak BgA subgroups is scarce and based on investigations of relatively few donors. An improved high-throughput flow cytometric assay was employed to investigate rosetting characteristics in an extensive panel of RBC donor samples of all four major ABO Bgs, as well as low BgA expressing samples. All non-O Bgs shield the parasite surface antigens from strain-specific antibodies towards P. falciparum erythrocyte membrane protein 1 (PfEMP1). A positive correlation between A-antigen levels on RBCs and rosette tightness was observed, protecting the rosettes from heparin- and antibody-mediated disruption. These results provide new insights into how the ABO Bg system affects the disease outcome and cautions against interpreting the results from the heterogeneous BgA phenotype as a single group in epidemiological and experimental studies.
Sections du résumé
BACKGROUND
BACKGROUND
The histo-blood group ABO system has been associated with adverse outcomes in COVID-19, thromboembolic diseases and Plasmodium falciparum malaria. An integral part of the severe malaria pathogenesis is rosetting, the adherence of parasite infected red blood cells (RBCs) to uninfected RBCs. Rosetting is influenced by the host's ABO blood group (Bg) and rosettes formed in BgA have previously been shown to be more resilient to disruption by heparin and shield the parasite derived surface antigens from antibodies. However, data on rosetting in weak BgA subgroups is scarce and based on investigations of relatively few donors.
METHODS
METHODS
An improved high-throughput flow cytometric assay was employed to investigate rosetting characteristics in an extensive panel of RBC donor samples of all four major ABO Bgs, as well as low BgA expressing samples.
RESULTS
RESULTS
All non-O Bgs shield the parasite surface antigens from strain-specific antibodies towards P. falciparum erythrocyte membrane protein 1 (PfEMP1). A positive correlation between A-antigen levels on RBCs and rosette tightness was observed, protecting the rosettes from heparin- and antibody-mediated disruption.
CONCLUSIONS
CONCLUSIONS
These results provide new insights into how the ABO Bg system affects the disease outcome and cautions against interpreting the results from the heterogeneous BgA phenotype as a single group in epidemiological and experimental studies.
Identifiants
pubmed: 34794445
doi: 10.1186/s12936-021-03975-w
pii: 10.1186/s12936-021-03975-w
pmc: PMC8600353
doi:
Substances chimiques
ABO Blood-Group System
0
Antibodies, Protozoan
0
Protozoan Proteins
0
erythrocyte membrane protein 1, Plasmodium falciparum
0
Heparin
9005-49-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
441Subventions
Organisme : Knut och Alice Wallenbergs Stiftelse
ID : KAW 2017.0055
Organisme : Knut och Alice Wallenbergs Stiftelse
ID : KAW 2017.0055
Organisme : Vetenskapsrådet
ID : VR 2018-05814
Organisme : Vetenskapsrådet
ID : VR 2018-05814
Organisme : Vetenskapsrådet
ID : VR 2016-02917
Organisme : Stiftelsen för Strategisk Forskning
ID : SB12-0026
Informations de copyright
© 2021. The Author(s).
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