Label-free detection of SARS-CoV-2 Spike S1 antigen triggered by electroactive gold nanoparticles on antibody coated fluorine-doped tin oxide (FTO) electrode.
Gold nanoparticles
Immunosensor
SARS-CoV-2
Spike antigen
Voltammetry
Journal
Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534
Informations de publication
Date de publication:
15 Dec 2021
15 Dec 2021
Historique:
received:
02
09
2021
revised:
04
10
2021
accepted:
19
10
2021
entrez:
19
11
2021
pubmed:
20
11
2021
medline:
23
11
2021
Statut:
ppublish
Résumé
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, also known as 2019-nCov or COVID-19) outbreak has become a huge public health issue due to its rapid transmission making it a global pandemic. Here, we report fabricated fluorine doped tin oxide (FTO) electrodes/gold nanoparticles (AuNPs) complex coupled with in-house developed SARS-CoV-2 spike S1 antibody (SARS-CoV-2 Ab) to measure the response with Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). The biophysical characterisation of FTO/AuNPs/SARS-CoV-2Ab was done via UV-Visible spectroscopy, Dynamic Light Scattering (DLS), and Fourier Transform Infrared Spectroscopy (FT-IR). The fabricated FTO/AuNPs/SARS-CoV-2Ab immunosensor was optimised for response time, antibody concentration, temperature, and pH. Under optimum conditions, the FTO/AuNPs/Ab based immunosensor displayed high sensitivity with limit of detection (LOD) up to 0.63 fM in standard buffer and 120 fM in spiked saliva samples for detection of SARS-CoV-2 spike S1 antigen (Ag) with negligible cross reactivity Middle East Respiratory Syndrome (MERS) spike protein. The proposed FTO/AuNPs/SARS-CoV-2Ab based biosensor proved to be stable for up to 4 weeks and can be used as an alternative non-invasive diagnostic tool for the rapid, specific and sensitive detection of SARS-CoV-2 Spike Ag traces in clinical samples.
Identifiants
pubmed: 34794571
pii: S0003-2670(21)01033-3
doi: 10.1016/j.aca.2021.339207
pmc: PMC8529383
pii:
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
Tin Compounds
0
spike protein, SARS-CoV-2
0
Fluorine
284SYP0193
Gold
7440-57-5
stannic oxide
KM7N50LOS6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339207Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Biosens Bioelectron. 2019 Sep 15;141:111398
pubmed: 31176112
Bioimpacts. 2018;8(1):53-58
pubmed: 29713602
Nat Biotechnol. 2020 Jul;38(7):870-874
pubmed: 32300245
Small. 2010 May 7;6(9):993-8
pubmed: 20394068
J Med Virol. 2020 Sep;92(9):1518-1524
pubmed: 32104917
Front Biosci (Landmark Ed). 2020 Jun 1;25(10):1875-1893
pubmed: 32472762
ACS Appl Bio Mater. 2021 Apr 19;4(4):2974-2995
pubmed: 35014387
Sci Rep. 2020 Sep 3;10(1):14546
pubmed: 32884083
Biosens Bioelectron. 2019 Feb 1;126:792-799
pubmed: 30557838
Lancet. 2020 Feb 15;395(10223):514-523
pubmed: 31986261
Biosens Bioelectron. 2005 Apr 15;20(10):1939-45
pubmed: 15741061
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Anal Chem. 2004 Jun 15;76(12):3285-98
pubmed: 15193109
Nat Commun. 2020 Sep 18;11(1):4711
pubmed: 32948757
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
RSC Adv. 2018 Jun 26;8(41):23163-23170
pubmed: 35540121
Polymers (Basel). 2020 Mar 27;12(4):
pubmed: 32230802
ACS Nano. 2020 Jun 23;14(6):7617-7627
pubmed: 32437124
ACS Nano. 2020 May 26;14(5):5268-5277
pubmed: 32281785
J Biomol Struct Dyn. 2018 Aug;36(10):2637-2653
pubmed: 28793850
Radiology. 2020 Aug;296(2):E46-E54
pubmed: 32155105
Microb Biotechnol. 2020 Jul;13(4):950-961
pubmed: 32333644
Clin Chem. 2021 Jan 30;67(2):415-424
pubmed: 33098427
Mater Lett. 2021 Dec 15;305:130824
pubmed: 36540867
Emerg Microbes Infect. 2020 Dec;9(1):998-1007
pubmed: 32306853
ACS Nano. 2020 Apr 28;14(4):5135-5142
pubmed: 32293168
Biosens Bioelectron. 2003 Oct 15;18(12):1429-37
pubmed: 12941557
Biosens Bioelectron. 2020 Sep 15;164:112316
pubmed: 32553350
RSC Adv. 2018 Jan 4;8(3):1511-1518
pubmed: 35540925
Radiology. 2020 Jun;295(3):200463
pubmed: 32077789
Chin Med J (Engl). 2020 May 5;133(9):1015-1024
pubmed: 32004165
AJR Am J Roentgenol. 2020 Jun;214(6):1280-1286
pubmed: 32130038
Anal Chem. 2021 Sep 28;93(38):13063-13071
pubmed: 34541844
Nano Lett. 2016 Jun 8;16(6):3668-74
pubmed: 27219521
Emerg Microbes Infect. 2020 Dec;9(1):313-319
pubmed: 32020836
Carbohydr Polym. 2021 Aug 15;266:118138
pubmed: 34044952
RSC Adv. 2020 Mar 24;10(20):11843-11850
pubmed: 35496625
Biosens Bioelectron. 2018 May 15;105:14-21
pubmed: 29346076
Sci Rep. 2020 Mar 13;10(1):4627
pubmed: 32170077
Radiology. 2020 Aug;296(2):E32-E40
pubmed: 32101510
Radiology. 2020 Aug;296(2):E115-E117
pubmed: 32073353
Eur Radiol. 2020 Jun;30(6):3306-3309
pubmed: 32055945
ACS Appl Bio Mater. 2020 Nov 16;3(11):7326-7343
pubmed: 35019474
J Med Virol. 2020 Apr;92(4):424-432
pubmed: 31981224
J Proteome Res. 2020 Nov 6;19(11):4389-4392
pubmed: 32568543
J Mater Chem B. 2021 Jun 16;9(23):4620-4642
pubmed: 34027540
Nanomedicine. 2019 Jun;18:196-220
pubmed: 30904587
Clin Chem. 2020 Apr 1;66(4):549-555
pubmed: 32031583
Emerg Microbes Infect. 2020 Dec;9(1):747-756
pubmed: 32196430
JAMA. 2020 Apr 21;323(15):1502-1503
pubmed: 32105304
Radiology. 2020 Jun;295(3):715-721
pubmed: 32053470
Diagnostics (Basel). 2020 Mar 18;10(3):
pubmed: 32197339
Nature. 2020 Mar;579(7798):265-269
pubmed: 32015508