Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 11 2021
18 11 2021
Historique:
received:
26
05
2021
accepted:
27
10
2021
entrez:
19
11
2021
pubmed:
20
11
2021
medline:
24
2
2022
Statut:
epublish
Résumé
Regenerative cartilage prepared from cultured chondrocytes is generally immature in vitro and matures after transplantation. Although many factors, including host cells and humoral factors, have been shown to affect cartilage maturation in vivo, the requirement of direct cell-cell contact between host and donor cells remains to be verified. In this study, we examined the host cells that promote cartilage maturation via cell-cell contact. Based on analysis of the transplanted chondrocytes, we examined the contribution of endothelial cells and macrophages. Using a semiclosed device that is permeable to tissue fluids while blocking host cells, we selectively transplanted chondrocytes and HUVECs or untreated/M1-polarized/M2-polarized RAW264.7 cells. As a result, untreated RAW264.7 cells induced cartilage regeneration. Furthermore, an in vitro coculture assay indicated communication between chondrocytes and RAW264.7 cells mediated by RNA, suggesting the involvement of extracellular vesicles in this process. These findings provide insights for establishing a method of in vitro cartilage regeneration.
Identifiants
pubmed: 34795319
doi: 10.1038/s41598-021-01437-6
pii: 10.1038/s41598-021-01437-6
pmc: PMC8602279
doi:
Substances chimiques
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
22476Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP18K09738
Organisme : Japan Society for the Promotion of Science
ID : JP21H03136
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2021. The Author(s).
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