Enhanced epigenetic profiling of classical human monocytes reveals a specific signature of healthy aging in the DNA methylome.
Journal
Nature aging
ISSN: 2662-8465
Titre abrégé: Nat Aging
Pays: United States
ID NLM: 101773306
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
entrez:
19
11
2021
pubmed:
20
11
2021
medline:
20
11
2021
Statut:
ppublish
Résumé
The impact of healthy aging on molecular programming of immune cells is poorly understood. Here, we report comprehensive characterization of healthy aging in human classical monocytes, with a focus on epigenomic, transcriptomic, and proteomic alterations, as well as the corresponding proteomic and metabolomic data for plasma, using healthy cohorts of 20 young and 20 older males (~27 and ~64 years old on average). For each individual, we performed eRRBS-based DNA methylation profiling, which allowed us to identify a set of age-associated differentially methylated regions (DMRs) - a novel, cell-type specific signature of aging in DNA methylome. Hypermethylation events were associated with H3K27me3 in the CpG islands near promoters of lowly-expressed genes, while hypomethylated DMRs were enriched in H3K4me1 marked regions and associated with age-related increase of expression of the corresponding genes, providing a link between DNA methylation and age-associated transcriptional changes in primary human cells.
Identifiants
pubmed: 34796338
doi: 10.1038/s43587-020-00002-6
pmc: PMC8597198
mid: NIHMS1635539
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
124-141Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR070811
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM125504
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR000448
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI105097
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002345
Pays : United States
Organisme : NIA NIH HHS
ID : P01 AG051459
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE028296
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA091842
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI105097
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM128802
Pays : United States
Commentaires et corrections
Type : CommentIn
Déclaration de conflit d'intérêts
COMPETING INTERESTS The authors declare no competing interests
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