C-terminal modified Enkephalin-like tetrapeptides with enhanced affinities at the kappa opioid receptor and monoamine transporters.


Journal

Bioorganic & medicinal chemistry
ISSN: 1464-3391
Titre abrégé: Bioorg Med Chem
Pays: England
ID NLM: 9413298

Informations de publication

Date de publication:
01 12 2021
Historique:
received: 12 07 2021
revised: 11 10 2021
accepted: 15 10 2021
pubmed: 20 11 2021
medline: 19 1 2022
entrez: 19 11 2021
Statut: ppublish

Résumé

A new series of enkephalin-like tetrapeptide analogs modified at the C-terminus by an N-(3,4-dichlorophenyl)-N-(piperidin-4-yl)propionamide (DPP) moiety were designed, synthesized, and tested for their binding affinities at opioid receptors and monoamine transporters to evaluate their potential multifunctional activity for the treatment of chronic pain. Most ligands exhibited high binding affinities in the nanomolar range at the opioid receptors with a slight delta-opioid receptor (DOR) selectivity over mu-opioid receptor (MOR) and kappa-opioid receptor (KOR) and low binding affinities in the micromolar range at the monoamine transporters, SERT and NET. Ligands of which the positions 1 and 4 were substituted by Dmt and Phe(4-X) residues, respectively, showed the excellent binding affinities at three opioid receptors. Among them, Dmt-d-Tic-Gly-Phe(4-F)-DPP was the most promising considering its excellent opioid affinities, particularly unexpected high binding affinity (Ki = 0.13 nM) at the KOR, and moderate interactions with serotonin/norepinephrine reuptake inhibitors (SNRIs). Docking studies revealed that the ligand was a good fit for the KOR binding pocket (binding score = 8,750).

Identifiants

pubmed: 34798381
pii: S0968-0896(21)00517-4
doi: 10.1016/j.bmc.2021.116509
pmc: PMC8649046
mid: NIHMS1759033
pii:
doi:

Substances chimiques

Amides 0
Ligands 0
Oligopeptides 0
Receptors, Opioid, kappa 0
Receptors, Opioid, mu 0
propionamide QK07G0HP47

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

116509

Subventions

Organisme : NIDA NIH HHS
ID : P01 DA006284
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

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Auteurs

School of Chemistry, University of the Punjab, Lahore 54590, Pakistan; Department of Chemistry, The University of Lahore, Lahore 54792, Pakistan.

Munawar A Munawar (MA)

School of Chemistry, University of the Punjab, Lahore 54590, Pakistan.

David Rankin (D)

Department of Pharmacology, University of Arizona, Tucson, AZ 85724, Unites States.

Victor J Hruby (VJ)

Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, Unites States.

Frank Porreca (F)

Department of Pharmacology, University of Arizona, Tucson, AZ 85724, Unites States.

Yeon Sun Lee (YS)

Department of Pharmacology, University of Arizona, Tucson, AZ 85724, Unites States. Electronic address: yeon@email.arizona.edu.

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Classifications MeSH