Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Mitochondria
complexome
copy numbers
disease
half-lives
high-confidence proteome
human cells
protein translocation
respiratory chain
smORFs
Journal
Cell metabolism
ISSN: 1932-7420
Titre abrégé: Cell Metab
Pays: United States
ID NLM: 101233170
Informations de publication
Date de publication:
07 12 2021
07 12 2021
Historique:
received:
12
05
2020
revised:
01
09
2021
accepted:
01
11
2021
pubmed:
21
11
2021
medline:
8
4
2022
entrez:
20
11
2021
Statut:
ppublish
Résumé
Mitochondria are key organelles for cellular energetics, metabolism, signaling, and quality control and have been linked to various diseases. Different views exist on the composition of the human mitochondrial proteome. We classified >8,000 proteins in mitochondrial preparations of human cells and defined a mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP). We identified interactors of translocases, respiratory chain, and ATP synthase assembly factors. The abundance of MitoCoP proteins covers six orders of magnitude and amounts to 7% of the cellular proteome with the chaperones HSP60-HSP10 being the most abundant mitochondrial proteins. MitoCoP dynamics spans three orders of magnitudes, with half-lives from hours to months, and suggests a rapid regulation of biosynthesis and assembly processes. 460 MitoCoP genes are linked to human diseases with a strong prevalence for the central nervous system and metabolism. MitoCoP will provide a high-confidence resource for placing dynamics, functions, and dysfunctions of mitochondria into the cellular context.
Identifiants
pubmed: 34800366
pii: S1550-4131(21)00529-5
doi: 10.1016/j.cmet.2021.11.001
pmc: PMC8664129
pii:
doi:
Substances chimiques
Mitochondrial Proteins
0
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2464-2483.e18Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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