The protective effects of quercetin nano-emulsion on intestinal mucositis induced by 5-fluorouracil in mice.
Animals
Antioxidants
/ chemistry
Catalase
/ metabolism
Emulsions
/ chemistry
Fluorouracil
Gene Expression
/ drug effects
Hypoxia-Inducible Factor 1, alpha Subunit
/ genetics
Intestinal Mucosa
/ drug effects
Male
Malondialdehyde
/ metabolism
Mice
Mucositis
/ chemically induced
NF-kappa B
/ genetics
Nanoparticles
/ chemistry
Oxidative Stress
/ drug effects
Protective Agents
/ pharmacology
Quercetin
/ chemistry
5- fluorouracil
Intestine
Mucositis
Nano-emulsion
Quercetin
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
31 12 2021
31 12 2021
Historique:
received:
18
09
2021
revised:
25
10
2021
accepted:
01
11
2021
pubmed:
21
11
2021
medline:
4
1
2022
entrez:
20
11
2021
Statut:
ppublish
Résumé
Intestinal mucositis is one of chemotherapeutics' most common adverse effects, such as 5-fluorouracil (5-FU). Quercetin (QRC), a naturally occurring flavonoid, has approved antioxidant and anti-inflammatory properties. Thus, in this article, the preventive and curative effects of emulsion and nano-emulsion formulations of QRC were investigated in a model of 5-FU-induced intestinal mucositis using biochemical, histopathological, and molecular approaches. Thirty-six mice were divided into six different groups: Control (normal saline), 5-FU (a single dose of 5-FU 300 mg/kg), pre-treatment groups (pre-QRC, and pre-QRC-nano, receiving QRC 5 mg/kg emulsion and nano-emulsion before the induction of mucositis, respectively), and post-treatment groups (post-QRC, and post-QRC-nano, receiving QRC 5 mg/kg emulsion and nano-emulsion after the induction of mucositis, respectively). The administration of quercetin emulsion and nano-emulsion could significantly alleviate the oxidant-antioxidant balance of mice serum samples and reverse the destructive histopathologic changes induced by 5-FU in the intestine tissue. Nevertheless, although the expression of both pro-inflammatory genes, NF-κB and HIF-1α, was decreased when quercetin was administered to mice, this reduction was not statistically significant. The administration of quercetin emulsion and nano-emulsion formulations could ameliorate the oxidative damage induced by chemotherapeutics, such as the 5-FU. Therefore, if confirmed in further studies, it could be used in clinical settings as a preventive and curative agent to decrease such catastrophic adverse events in chemotherapy patients.
Sections du résumé
BACKGROUND
Intestinal mucositis is one of chemotherapeutics' most common adverse effects, such as 5-fluorouracil (5-FU). Quercetin (QRC), a naturally occurring flavonoid, has approved antioxidant and anti-inflammatory properties. Thus, in this article, the preventive and curative effects of emulsion and nano-emulsion formulations of QRC were investigated in a model of 5-FU-induced intestinal mucositis using biochemical, histopathological, and molecular approaches.
METHOD
Thirty-six mice were divided into six different groups: Control (normal saline), 5-FU (a single dose of 5-FU 300 mg/kg), pre-treatment groups (pre-QRC, and pre-QRC-nano, receiving QRC 5 mg/kg emulsion and nano-emulsion before the induction of mucositis, respectively), and post-treatment groups (post-QRC, and post-QRC-nano, receiving QRC 5 mg/kg emulsion and nano-emulsion after the induction of mucositis, respectively).
FINDING
The administration of quercetin emulsion and nano-emulsion could significantly alleviate the oxidant-antioxidant balance of mice serum samples and reverse the destructive histopathologic changes induced by 5-FU in the intestine tissue. Nevertheless, although the expression of both pro-inflammatory genes, NF-κB and HIF-1α, was decreased when quercetin was administered to mice, this reduction was not statistically significant.
CONCLUSION
The administration of quercetin emulsion and nano-emulsion formulations could ameliorate the oxidative damage induced by chemotherapeutics, such as the 5-FU. Therefore, if confirmed in further studies, it could be used in clinical settings as a preventive and curative agent to decrease such catastrophic adverse events in chemotherapy patients.
Identifiants
pubmed: 34800883
pii: S0006-291X(21)01508-4
doi: 10.1016/j.bbrc.2021.11.005
pii:
doi:
Substances chimiques
Antioxidants
0
Emulsions
0
Hypoxia-Inducible Factor 1, alpha Subunit
0
NF-kappa B
0
Protective Agents
0
Malondialdehyde
4Y8F71G49Q
Quercetin
9IKM0I5T1E
Catalase
EC 1.11.1.6
Fluorouracil
U3P01618RT
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
75-81Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no conflict of interest.