Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida.
Cross-protection
Deletion
Quorum sensing gene
Vaccine
Virulence
Journal
Veterinary research
ISSN: 1297-9716
Titre abrégé: Vet Res
Pays: England
ID NLM: 9309551
Informations de publication
Date de publication:
20 Nov 2021
20 Nov 2021
Historique:
received:
08
09
2021
accepted:
18
10
2021
entrez:
21
11
2021
pubmed:
22
11
2021
medline:
28
12
2021
Statut:
epublish
Résumé
QseC, a histidine sensor kinase of the QseBC two-component system, acts as a global regulator of bacterial stress resistance, biofilm formation, and virulence. The function of QseC in some bacteria is well understood, but not in Pasteurella multocida. We found that deleting qseC in P. multocida serotype A:L3 significantly down-regulated bacterial virulence. The mutant had significantly reduced capsule production but increased resistance to oxidative stress and osmotic pressure. Deleting qseC led to a significant increase in qseB expression. Transcriptome sequencing analysis showed that 1245 genes were regulated by qseC, primarily those genes involved in capsule and LPS biosynthesis and export, biofilm formation, and iron uptake/utilization, as well as several immuno-protection related genes including ompA, ptfA, plpB, vacJ, and sodA. In addition to presenting strong immune protection against P. multocida serotypes A:L1 and A:L3 infection, live ΔqseC also exhibited protection against P. multocida serotype B:L2 and serotype F:L3 infection in a mouse model. The results indicate that QseC regulates capsular production and virulence in P. multocida. Furthermore, the qseC mutant can be used as an attenuated vaccine against P. multocida strains of multiple serotypes.
Identifiants
pubmed: 34801081
doi: 10.1186/s13567-021-01009-6
pii: 10.1186/s13567-021-01009-6
pmc: PMC8605557
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
140Subventions
Organisme : Natural Science Foundation of Chongqing
ID : cstc2017jcyjAX0288
Organisme : China Agriculture Research System of MOF and MARA
ID : CARS-37
Informations de copyright
© 2021. The Author(s).
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