First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa.
Anopheles arabiensis
Kdr mutation
Knockdown resistance
Malaria
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
21 Nov 2021
21 Nov 2021
Historique:
received:
05
08
2021
accepted:
08
11
2021
entrez:
22
11
2021
pubmed:
23
11
2021
medline:
13
1
2022
Statut:
epublish
Résumé
Due to the lack of vaccines, malaria control mainly involves the control of anopheline vectors (Anopheles spp.) using chemical insecticides. However, the prolonged and indiscriminate use of these compounds has led to the emergence of resistance in Anopheles populations in Africa. Insecticide resistance surveillance programs are less frequent in Cabo Verde than in other African countries. This study aimed to investigate the circulation of the L1014F and L1014S alleles in natural populations of Anopheles arabiensis collected from two sampling sites in the city of Praia, Cabo Verde. Anopheles larvae were collected from the two sampling sites and reared in the laboratory until the adult stage. Mosquitoes were first morphologically identified by classical taxonomy and then by molecular species identification using molecular markers. All Anopheles arabiensis were subjected to PCR analysis to screen for mutations associated to resistance in the Na A total of 105 mosquitoes, all belonging to the Anopheles gambiae complex, were identified by classical taxonomy as well as by molecular taxonomy. Molecular identification showed that 100% of the An. gambiae senso lato specimens analyzed corresponded to An. arabiensis. Analysis of the Na Our data demonstrated, for the first time, the presence of the L1014F allele in the An. arabiensis population from Cabo Verde, as well as an increase in the frequency of the kdr L1014S allele reported in a previous study. The results of this study demonstrate the need to establish new approaches in vector control programs in Cabo Verde.
Sections du résumé
BACKGROUND
BACKGROUND
Due to the lack of vaccines, malaria control mainly involves the control of anopheline vectors (Anopheles spp.) using chemical insecticides. However, the prolonged and indiscriminate use of these compounds has led to the emergence of resistance in Anopheles populations in Africa. Insecticide resistance surveillance programs are less frequent in Cabo Verde than in other African countries. This study aimed to investigate the circulation of the L1014F and L1014S alleles in natural populations of Anopheles arabiensis collected from two sampling sites in the city of Praia, Cabo Verde.
METHODS
METHODS
Anopheles larvae were collected from the two sampling sites and reared in the laboratory until the adult stage. Mosquitoes were first morphologically identified by classical taxonomy and then by molecular species identification using molecular markers. All Anopheles arabiensis were subjected to PCR analysis to screen for mutations associated to resistance in the Na
RESULTS
RESULTS
A total of 105 mosquitoes, all belonging to the Anopheles gambiae complex, were identified by classical taxonomy as well as by molecular taxonomy. Molecular identification showed that 100% of the An. gambiae senso lato specimens analyzed corresponded to An. arabiensis. Analysis of the Na
CONCLUSIONS
CONCLUSIONS
Our data demonstrated, for the first time, the presence of the L1014F allele in the An. arabiensis population from Cabo Verde, as well as an increase in the frequency of the kdr L1014S allele reported in a previous study. The results of this study demonstrate the need to establish new approaches in vector control programs in Cabo Verde.
Identifiants
pubmed: 34802463
doi: 10.1186/s13071-021-05088-4
pii: 10.1186/s13071-021-05088-4
pmc: PMC8607584
doi:
Substances chimiques
Insecticides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
582Subventions
Organisme : coordenação de aperfeiçoamento de pessoal de nível superior
ID : 0021/13
Organisme : fundação de amparo à ciência e tecnologia do estado de pernambuco
ID : APQ-0085-2.13/16
Informations de copyright
© 2021. The Author(s).
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