Respiratory and Intramuscular Immunization With ChAdOx2-NPM1-NA Induces Distinct Immune Responses in H1N1pdm09 Pre-Exposed Pigs.
Adenoviridae
/ genetics
Aerosols
Animals
Antibodies, Viral
/ blood
Cytokines
/ biosynthesis
Influenza A Virus, H1N1 Subtype
/ immunology
Influenza Vaccines
/ administration & dosage
Neuraminidase
/ immunology
Nucleocapsid Proteins
/ immunology
Swine
Vaccination
Viral Matrix Proteins
/ immunology
Virus Shedding
aerosol
influenza A
intramuscular
intranasal
pdmH1N1
pig
pre-exposure
vaccine
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
08
2021
accepted:
13
10
2021
entrez:
22
11
2021
pubmed:
23
11
2021
medline:
11
2
2022
Statut:
epublish
Résumé
There is a critical need to develop superior influenza vaccines that provide broader protection. Influenza vaccines are traditionally tested in naive animals, although humans are exposed to influenza in the first years of their lives, but the impact of prior influenza exposure on vaccine immune responses has not been well studied. Pigs are an important natural host for influenza, are a source of pandemic viruses, and are an excellent model for human influenza. Here, we investigated the immunogenicity of the ChAdOx2 viral vectored vaccine, expressing influenza nucleoprotein, matrix protein 1, and neuraminidase in H1N1pdm09 pre-exposed pigs. We evaluated the importance of the route of administration by comparing intranasal, aerosol, and intramuscular immunizations. Aerosol delivery boosted the local lung T-cell and antibody responses, while intramuscular immunization boosted peripheral blood immunity. These results will inform how best to deliver vaccines in order to harness optimal protective immunity.
Identifiants
pubmed: 34804053
doi: 10.3389/fimmu.2021.763912
pmc: PMC8595216
doi:
Substances chimiques
Aerosols
0
Antibodies, Viral
0
Cytokines
0
Influenza Vaccines
0
M1 protein, Influenza A virus
0
Nucleocapsid Proteins
0
Viral Matrix Proteins
0
Neuraminidase
EC 3.2.1.18
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
763912Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007031
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007039
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S037160/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007037
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S506680/1
Pays : United Kingdom
Informations de copyright
Copyright © 2021 Vatzia, Allen, Manjegowda, Morris, McNee, Martini, Kaliath, Ulaszewska, Boyd, Paudyal, Carr, Chrun, Maze, MacLoughlin, van Diemen, Everett, Lambe, Gilbert and Tchilian.
Déclaration de conflit d'intérêts
SG is co-founder of Vaccitech and named as an inventor on a patent covering use of ChAdOx2-vectored vaccines. TL is a consultant to Vaccitech. RM is an employee of Aerogen Limited and is a named inventor on inhaled vaccine delivery system patents. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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