Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography.
European X-ray Free-Electron Laser
X-ray crystallography
antibiotic resistance
ceftriaxone
drug discovery
enzyme kinetics
enzyme mechanisms
irreversible inhibition
megahertz pulse-repetition rate
mix-and-inject serial crystallography
protein structure determination
serial femtosecond crystallography
substrate diffusion in crystals
sulbactam
β-lactamases
Journal
IUCrJ
ISSN: 2052-2525
Titre abrégé: IUCrJ
Pays: England
ID NLM: 101623101
Informations de publication
Date de publication:
01 Nov 2021
01 Nov 2021
Historique:
received:
21
06
2021
accepted:
06
08
2021
entrez:
22
11
2021
pubmed:
23
11
2021
medline:
23
11
2021
Statut:
epublish
Résumé
Here, we illustrate what happens inside the catalytic cleft of an enzyme when substrate or ligand binds on single-millisecond timescales. The initial phase of the enzymatic cycle is observed with near-atomic resolution using the most advanced X-ray source currently available: the European XFEL (EuXFEL). The high repetition rate of the EuXFEL combined with our mix-and-inject technology enables the initial phase of ceftriaxone binding to the
Identifiants
pubmed: 34804542
doi: 10.1107/S2052252521008125
pii: S2052252521008125
pmc: PMC8562667
doi:
Types de publication
Journal Article
Langues
eng
Pagination
878-895Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM095583
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM117342
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008267
Pays : United States
Informations de copyright
© Suraj Pandey et al. 2021.
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