Cyclodextrins Exert a Ligand-like Current Inhibitory Effect on the K
KV1.3
cholesterol
cyclodextrin
ligand-like interaction
membrane fluidity
membrane hydration
membrane lipid order
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2021
2021
Historique:
received:
02
07
2021
accepted:
19
10
2021
entrez:
22
11
2021
pubmed:
23
11
2021
medline:
23
11
2021
Statut:
epublish
Résumé
Cyclodextrins (CDs) are cyclic oligosaccharides capable of forming water-soluble complexes with a variety of otherwise poorly soluble molecules including cholesterol and different drugs. Consistently, CDs are widely used in research and clinical practice to deplete cholesterol from cellular membranes or to increase solubility and bioavailability of different pharmaceuticals at local concentrations in the millimolar range. Effects of CDs exerted on cellular functions are generally thought to originate from reductions in cholesterol levels. Potential direct, ligand-like CD effects are largely neglected in spite of several recent studies reporting direct interaction between CDs and proteins including AMP-activated protein kinase, β-amyloid peptides, and α-synuclein. In this study, by using patch-clamp technique, time-resolved quantitation of cholesterol levels and biophysical parameters and applying cholesterol-extracting and non-cholesterol-extracting CDs at 1 and 5 mM concentrations, we provide evidence for a previously unexplored ligand-like, cholesterol-independent current inhibitory effect of CDs on K
Identifiants
pubmed: 34805269
doi: 10.3389/fmolb.2021.735357
pii: 735357
pmc: PMC8599428
doi:
Types de publication
Journal Article
Langues
eng
Pagination
735357Informations de copyright
Copyright © 2021 Kovacs, Sohajda, Szente, Nagy, Panyi, Varga and Zakany.
Déclaration de conflit d'intérêts
TS and LS was employed by the CycloLab Cyclodextrin R and D Laboratory Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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