Frizzled3 inhibits Vangl2-Prickle3 association to establish planar cell polarity in the vertebrate neural plate.
Xenopus
Neuroectoderm
PCP
Phosphorylation
Prickle3
Vangl2
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
15 12 2021
15 12 2021
Historique:
received:
04
05
2021
accepted:
09
11
2021
pubmed:
23
11
2021
medline:
3
2
2022
entrez:
22
11
2021
Statut:
ppublish
Résumé
The orientation of epithelial cells in the plane of the tissue, known as planar cell polarity (PCP), is regulated by interactions of asymmetrically localized PCP protein complexes. In the Xenopus neural plate, Van Gogh-like2 (Vangl2) and Prickle3 (Pk3) proteins form a complex at the anterior cell boundaries, but how this complex is regulated in vivo remains largely unknown. Here, we use proximity biotinylation and crosslinking approaches to show that Vangl2-Pk3 association is inhibited by Frizzled3 (Fz3, also known as Fzd3), a core PCP protein that is specifically expressed in the neuroectoderm and is essential for the establishment of PCP in this tissue. This inhibition required Fz3-dependent Vangl2 phosphorylaton. Consistent with our observations, the complex of Pk3 with nonphosphorylatable Vangl2 did not polarize in the neural plate. These findings provide evidence for in vivo regulation of Vangl2-Pk3 complex formation and localization by a Frizzled receptor.
Identifiants
pubmed: 34806749
pii: 273708
doi: 10.1242/jcs.258864
pmc: PMC8729781
pii:
doi:
Substances chimiques
FZD3 protein, Xenopus
0
Frizzled Receptors
0
Intercellular Signaling Peptides and Proteins
0
Membrane Proteins
0
Transcription Factors
0
Vangl2 protein, Xenopus
0
Xenopus Proteins
0
prickle3 protein, Xenopus
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : R35GM122492
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS100759
Pays : United States
Organisme : NIH HHS
ID : NS100759
Pays : United States
Organisme : NIH HHS
ID : GM122492
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122492
Pays : United States
Informations de copyright
© 2021. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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