Engineering Cupriavidus necator DSM 545 for the one-step conversion of starchy waste into polyhydroxyalkanoates.
3HB
Broken rice
Consolidated BioProcessing
Recombinant amylase expression
Sweet potato waste
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
08
10
2021
revised:
15
11
2021
accepted:
16
11
2021
pubmed:
23
11
2021
medline:
17
2
2022
entrez:
22
11
2021
Statut:
ppublish
Résumé
Starch-rich by-products could be efficiently exploited for polyhydroxyalkanoates (PHAs) production. Unfortunately, Cupriavidus necator DSM 545, one of the most efficient PHAs producers, is not able to grow on starch. In this study, a recombinant amylolytic strain of C. necator DSM 545 was developed for the one-step PHAs production from starchy residues, such as broken rice and purple sweet potato waste. The glucodextranase G1d from Arthrobacter globiformis I42 and the α-amylase amyZ from Zunongwangia profunda SM-A87 were co-expressed into C. necator DSM 545. The recombinant C. necator DSM 545 #11, selected for its promising hydrolytic activity, produced high biomass levels with noteworthy PHAs titers: 5.78 and 3.65 g/L from broken rice and purple sweet potato waste, respectively. This is the first report on the engineering of C. necator DSM 545 for efficient amylase production and paves the way to the one-step conversion of starchy waste into PHAs.
Identifiants
pubmed: 34808314
pii: S0960-8524(21)01725-9
doi: 10.1016/j.biortech.2021.126383
pii:
doi:
Substances chimiques
Polyhydroxyalkanoates
0
Starch
9005-25-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126383Informations de copyright
Crown Copyright © 2021. Published by Elsevier Ltd. All rights reserved.