Non-apoptotic cell death such as pyroptosis, autophagy, necroptosis and ferroptosis acts as partners to induce testicular cell death after scrotal hyperthermia in mice.
autophagy
ferroptosis
necroptosis
pyroptosis
scrotal hyperthermia
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
04
11
2021
received:
02
07
2021
accepted:
11
11
2021
pubmed:
24
11
2021
medline:
16
2
2022
entrez:
23
11
2021
Statut:
ppublish
Résumé
Cell death is a biologically uncontrollable and regulated process associated with human diseases which usually occur in response to oxidative stress that activates signalling pathways in multiple forms and can therefore contribute to human diseases. Thus, the current study aims to evaluate the signalling pathway involved in cell death after testicular hyperthermia. For this purpose, 32 mice were equally divided into four groups; I: Control; II, III and IV, Scrotal hyperthermia in which the testes are exposed to water at 43°C for 20 min every other day, respectively, 15, 10 and 5 times. Then, animals were euthanized and testicular tissue samples were isolated to evaluate protein expression as well as germ cell gene marker expression by Western blot and real-time PCR tests. Our data showed that the protein expression of Caspase-1, Beclin1, Atg7, Mlkl and Acsl4 together with the expression of Caspase-1, Beclin1, Atg7, Mlkl and Acsl4 genes was significantly up-regulated in scrotal hyperthermia-induced mice. In conclusion, the present study showed that heat stress disrupts spermatogenesis by activating several non-apoptotic signalling pathways in testicular tissue.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14320Subventions
Organisme : Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
ID : 27068
Informations de copyright
© 2021 Wiley-VCH GmbH.
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