Evaluation of MMP-12 expression in chronic rhinosinusitis with nasal polyposis.
Journal
Rhinology
ISSN: 0300-0729
Titre abrégé: Rhinology
Pays: Netherlands
ID NLM: 0347242
Informations de publication
Date de publication:
01 Feb 2022
01 Feb 2022
Historique:
pubmed:
24
11
2021
medline:
22
2
2022
entrez:
23
11
2021
Statut:
ppublish
Résumé
The purpose of this study was to evaluate the expression of MMP-12 in patients with chronic rhinosinusitis with polyps (CRSwNP). Tissue samples from 37 patients with CRSwNP undergoing functional endoscopic sinus surgery and healthy mucosa specimens from 12 healthy controls were obtained intraoperatively. The mRNA and protein expression levels of MMP-12 were quantified by real-time polymerase chain reaction and Western blotting, respectively. mRNA levels of MMP-12 were significantly elevated in the CRSwNP tissue samples compared to those in control ones. The protein levels of MMP-12 showed a trend of increasing but with no statistical significance. Elevation of MMP-12 in patients with CRSwNP suggests its potential implication in the pathogenesis of the disease. The difference in the expression profile observed between mRNA and protein levels could be due to post-translational gene expression regulation. Our findings provide evidence that MMP-12 along with other MMPs may serve as a biomarker and therapeutic target in the management of the disease.
Sections du résumé
BACKGROUND
BACKGROUND
The purpose of this study was to evaluate the expression of MMP-12 in patients with chronic rhinosinusitis with polyps (CRSwNP).
METHODOLOGY
METHODS
Tissue samples from 37 patients with CRSwNP undergoing functional endoscopic sinus surgery and healthy mucosa specimens from 12 healthy controls were obtained intraoperatively. The mRNA and protein expression levels of MMP-12 were quantified by real-time polymerase chain reaction and Western blotting, respectively.
RESULTS
RESULTS
mRNA levels of MMP-12 were significantly elevated in the CRSwNP tissue samples compared to those in control ones. The protein levels of MMP-12 showed a trend of increasing but with no statistical significance.
CONCLUSIONS
CONCLUSIONS
Elevation of MMP-12 in patients with CRSwNP suggests its potential implication in the pathogenesis of the disease. The difference in the expression profile observed between mRNA and protein levels could be due to post-translational gene expression regulation. Our findings provide evidence that MMP-12 along with other MMPs may serve as a biomarker and therapeutic target in the management of the disease.
Identifiants
pubmed: 34812434
pii: 2951
doi: 10.4193/Rhin21.320
doi:
Substances chimiques
Matrix Metalloproteinases
EC 3.4.24.-
MMP12 protein, human
EC 3.4.24.65
Matrix Metalloproteinase 12
EC 3.4.24.65
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM