Cyclopentane peptide nucleic acid: Gold nanoparticle conjugates for the detection of nucleic acids in a microfluidic format.
Peptide nucleic acid
gold nanoparticles
microfluidics
nucleic acid detection
Journal
Biopolymers
ISSN: 1097-0282
Titre abrégé: Biopolymers
Pays: United States
ID NLM: 0372525
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
revised:
19
10
2021
received:
19
07
2021
accepted:
25
10
2021
pubmed:
24
11
2021
medline:
26
3
2022
entrez:
23
11
2021
Statut:
ppublish
Résumé
Routine patient testing for viral infections is critical to identify infected individuals for treatment and to prevent spreading of infections to others. Developing robust and reliable diagnostic tools to detect nucleic acids of viruses at the point-of-care could greatly assist the clinical management of viral infections. The remarkable stability and high binding affinity of peptide nucleic acids (PNAs) to target nucleic acids could make PNA-based biosensors an excellent starting point to develop new nucleic acid detection technologies. We report the application of cyclopentane-modified PNAs to capture target nucleic acids in a microfluidic channel, and the use of bioorthogonal PNAs conjugated to gold nanoparticles as probes to semi-quantitatively signal the presence of a target nucleic acid derived from HIV-1. The basic results presented could be used to develop more advanced devices to detect nucleic acids from viruses such as HIV, SARS-CoV-2, and a wide range of other human diseases.
Identifiants
pubmed: 34812507
doi: 10.1002/bip.23481
pmc: PMC8957522
mid: NIHMS1752587
doi:
Substances chimiques
Cyclopentanes
0
Nucleic Acids
0
Peptide Nucleic Acids
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23481Subventions
Organisme : Office of AIDS Research
Organisme : Intramural NIH HHS
ID : Z01 DK031119
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA DK031143
Pays : United States
Organisme : Intramural NIH HHS
ID : Z01 DK031143
Pays : United States
Organisme : NIBIB NIH HHS
Pays : United States
Organisme : NIH HHS
Pays : United States
Organisme : NIDDK NIH HHS
Pays : United States
Informations de copyright
© 2021 Wiley Periodicals LLC. This article has been contributed to by US Government employees and their work is in the public domain in the USA.
Références
AIDS. 2007 Jul;21 Suppl 4:S81-7
pubmed: 17620757
J Am Chem Soc. 2004 Nov 24;126(46):15067-73
pubmed: 15548003
Microfluid Nanofluidics. 2011;10(2):231-247
pubmed: 32214951
Biomicrofluidics. 2013 Jul 30;7(4):41501
pubmed: 24003344
Curr Opin Biotechnol. 1999 Feb;10(1):71-5
pubmed: 10047504
Yale J Biol Med. 2017 Dec 19;90(4):583-598
pubmed: 29259523
Org Lett. 2003 Jul 24;5(15):2695-8
pubmed: 12868892
ACS Nano. 2020 Apr 28;14(4):3822-3835
pubmed: 32223179
Science. 1991 Dec 6;254(5037):1497-500
pubmed: 1962210
Electrophoresis. 2002 Oct;23(20):3461-73
pubmed: 12412113
Nature. 1996 Aug 15;382(6592):607-9
pubmed: 8757129
J Am Chem Soc. 2004 Oct 20;126(41):13186-7
pubmed: 15479048
Nucleic Acids Res. 2021 Jan 25;49(2):713-725
pubmed: 33406227
Anal Chem. 2009 Aug 1;81(15):6122-9
pubmed: 20337394
J Infect Dis. 2010 Apr 15;201 Suppl 1:S42-5
pubmed: 20225945
Rev Med Virol. 2021 Jan;31(1):1-11
pubmed: 32844526
Nature. 2006 Jul 27;442(7101):368-73
pubmed: 16871203
Org Lett. 2018 Dec 7;20(23):7637-7640
pubmed: 30460846
Angew Chem Int Ed Engl. 2013 Apr 8;52(15):4217-20
pubmed: 23460137
Curr Opin Biotechnol. 2001 Feb;12(1):16-20
pubmed: 11167067
Proc Math Phys Eng Sci. 2020 Nov;476(2243):20200398
pubmed: 33363440
Org Lett. 2004 Dec 9;6(25):4699-702
pubmed: 15575664
J Biosci. 2008 Nov;33(4):617-28
pubmed: 19208986
Talanta. 2005 Sep 15;67(3):449-55
pubmed: 18970188