Micron-scale supramolecular myosin arrays help mediate cytoskeletal assembly at mature adherens junctions.
Actin Cytoskeleton
/ metabolism
Actin-Related Protein 2-3 Complex
/ metabolism
Actins
/ metabolism
Actomyosin
/ metabolism
Adherens Junctions
/ metabolism
Animals
Caco-2 Cells
Cytoskeleton
/ metabolism
DNA-Binding Proteins
Dogs
Formins
/ metabolism
Humans
Madin Darby Canine Kidney Cells
Models, Biological
Myosin-Light-Chain Kinase
/ metabolism
Myosins
/ metabolism
rho-Associated Kinases
/ metabolism
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
03 01 2022
03 01 2022
Historique:
received:
12
03
2021
revised:
28
09
2021
accepted:
14
10
2021
entrez:
23
11
2021
pubmed:
24
11
2021
medline:
24
12
2021
Statut:
ppublish
Résumé
Epithelial cells assemble specialized actomyosin structures at E-Cadherin-based cell-cell junctions, and the force exerted drives cell shape change during morphogenesis. The mechanisms that build this supramolecular actomyosin structure remain unclear. We used ZO-knockdown MDCK cells, which assemble a robust, polarized, and highly organized actomyosin cytoskeleton at the zonula adherens, combining genetic and pharmacologic approaches with superresolution microscopy to define molecular machines required. To our surprise, inhibiting individual actin assembly pathways (Arp2/3, formins, or Ena/VASP) did not prevent or delay assembly of this polarized actomyosin structure. Instead, as junctions matured, micron-scale supramolecular myosin arrays assembled, with aligned stacks of myosin filaments adjacent to the apical membrane, overlying disorganized actin filaments. This suggested that myosin arrays might bundle actin at mature junctions. Consistent with this idea, inhibiting ROCK or myosin ATPase disrupted myosin localization/organization and prevented actin bundling and polarization. We obtained similar results in Caco-2 cells. These results suggest a novel role for myosin self-assembly, helping drive actin organization to facilitate cell shape change.
Identifiants
pubmed: 34812842
pii: 212872
doi: 10.1083/jcb.202103074
pmc: PMC8614156
pii:
doi:
Substances chimiques
Actin-Related Protein 2-3 Complex
0
Actins
0
DNA-Binding Proteins
0
ENA-VASP proteins
0
Formins
0
Actomyosin
9013-26-7
rho-Associated Kinases
EC 2.7.11.1
Myosin-Light-Chain Kinase
EC 2.7.11.18
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : T32 CA009156
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118096
Pays : United States
Organisme : NIH HHS
ID : T32 CA009156
Pays : United States
Organisme : NIH HHS
ID : R35 GM118096
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM102390
Pays : United States
Organisme : NIH HHS
ID : R01 GM102390
Pays : United States
Informations de copyright
© 2021 Yu-Kemp et al.
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