Curing two predominant viruses occurring in Lentinula edodes by chemotherapy and mycelial fragmentation methods.

Fungal Viruses / genetics Mycelium RNA, Double-Stranded Shiitake Mushrooms
Lentinula edodes Mycelial fragmentation Mycovirus Ribavirin Virus curing

Journal

Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839

Informations de publication

Date de publication:
02 2022
Historique:
received: 29 02 2020
revised: 02 06 2021
accepted: 18 11 2021
pubmed: 24 11 2021
medline: 22 3 2022
entrez: 23 11 2021
Statut: ppublish

Résumé

Previous research has established that Lentinula edodes mycovirus HKB (LeV-HKB) and L. edodes partitivirus 1(LePV1) are major mycoviruses identified in L.edodes germplasm. In this paper, two different methods for curing these two dsRNA mycoviruses, ribavirin treatment and mycelial fragmentation, were evaluated for the first time. Mycelial fragmentation was found to resulted in LeV-HKB- and LePV1-cured fungal strains, whereas ribavirin treatment could eliminate LeV-HKB only. Although no LePV1-cured strain was obtained via ribavirin treatment by the end of the experiment, the relative LePV1 concentration in the eighth successive subcultures was lower than that of the untreated control. The culture features of several virus-cured strains had faster mycelial growth rate and higher colony density than the infected ones. It was also suggested that LeV-HKB infection may affect the pigmentation in plate- and bag-cultivated mycelia of L. edodes strain L135.

Identifiants

DOI: 10.1016/j.jviromet.2021.114370 PMID: 34813824
pubmed: 34813824
pii: S0166-0934(21)00309-8
doi: 10.1016/j.jviromet.2021.114370
pii:
doi:

Substances chimiques

RNA, Double-Stranded 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

114370

Informations de copyright

Copyright © 2021. Published by Elsevier B.V.

Auteurs

Yijia Sun (Y)

Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Mengpei Guo (M)

Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Jinjie Wang (J)

Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Yinbing Bian (Y)

Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Zhangyi Xu (Z)

Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture), Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address: zyxu2013@126.com.

Articles similaires

Inhibiting EZH2 targets atypical teratoid rhabdoid tumor by triggering viral mimicry via both RNA and DNA sensing pathways.

Shengrui Feng, Sajid A Marhon, Dustin J Sokolowski et al.
1.00
Enhancer of Zeste Homolog 2 Protein Humans Rhabdoid Tumor SMARCB1 Protein Cell Line, Tumor

RNA interference protocols for gene silencing in the spittlebug Philaenus spumarius, vector of Xylella fastidiosa.

Cecilia Parise, Luciana Galetto, Simona Abbà et al.
1.00
RNA Interference Xylella Animals Hemiptera Insect Vectors

Sprayable RNAi for silencing of important genes to manage red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae).

Muhammad Naeem Sattar, Muhammad Nadir Naqqash, Adel A Rezk et al.
1.00
Animals RNA Interference Weevils Larva RNA, Double-Stranded

The role of dsRNA A-to-I editing catalyzed by ADAR family enzymes in the pathogeneses.

Wanqing Liu, Yufan Wu, Tong Zhang et al.
1.00
Adenosine Deaminase Humans RNA Editing RNA, Double-Stranded Animals

Classifications MeSH

questionsmedicales.fr › Virus › Virus fongiques › Curing two predominant viruses occurring in...
questionsmedicales.fr › Structures fongiques › Mycelium › Curing two predominant viruses occurring in...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › ARN double brin › Curing two predominant viruses occurring in...
questionsmedicales.fr › Eucaryotes › Champignons › Basidiomycota › Agaricales › Lentinula › Champignons shiitake › Curing two predominant viruses occurring in...