Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 11 2021
23 11 2021
Historique:
received:
22
07
2021
accepted:
08
11
2021
entrez:
24
11
2021
pubmed:
25
11
2021
medline:
1
2
2022
Statut:
epublish
Résumé
Venous thromboembolism is a significant source of morbidity and mortality worldwide. Catheter-directed thrombolytics is the primary treatment used to relieve critical obstructions, though its efficacy varies based on the thrombus composition. Non-responsive portions of the specimen often remain in situ, which prohibits mechanistic investigation of lytic resistance or the development of diagnostic indicators for treatment outcomes. In this study, thrombus samples extracted from venous thromboembolism patients were analyzed ex vivo to determine their histological properties, susceptibility to lytic therapy, and imaging characteristics. A wide range of thrombus morphologies were observed, with a dependence on age and etymology of the specimen. Fibrinolytic inhibitors including PAI-1, alpha 2-antiplasmin, and TAFI were present in samples, which may contribute to the response venous thrombi to catheter-directed thrombolytics. Finally, a weak but significant correlation was observed between the response of the sample to lytic drug and its magnetic microstructure assessed with a quantitative MRI sequence. These findings highlight the myriad of changes in venous thrombi that may promote lytic resistance, and imaging metrics that correlate with treatment outcomes.
Identifiants
pubmed: 34815441
doi: 10.1038/s41598-021-02030-7
pii: 10.1038/s41598-021-02030-7
pmc: PMC8610976
doi:
Substances chimiques
Biomarkers
0
Fibrinolytic Agents
0
Tissue Plasminogen Activator
EC 3.4.21.68
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
22805Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL133334
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133334
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133334
Pays : United States
Informations de copyright
© 2021. The Author(s).
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