Fragmentation of tissue-resident macrophages during isolation confounds analysis of single-cell preparations from mouse hematopoietic tissues.

Animals Bone Marrow / metabolism Cell Separation / methods Hematopoiesis Hematopoietic Stem Cells / cytology Homeostasis Macrophages / cytology Mice Single-Cell Analysis / methods
RNA-seq bone marrow flow cytometry hematopoietic stem and progenitor cells hematopoietic tissues imaging flow cytometry lymph node macrophage spleen tissue-resident macrophage

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
23 11 2021
Historique:
received: 21 04 2021
revised: 28 09 2021
accepted: 03 11 2021
entrez: 24 11 2021
pubmed: 25 11 2021
medline: 15 2 2022
Statut: ppublish

Résumé

Mouse hematopoietic tissues contain abundant tissue-resident macrophages that support immunity, hematopoiesis, and bone homeostasis. A systematic strategy to characterize macrophage subsets in mouse bone marrow (BM), spleen, and lymph node unexpectedly reveals that macrophage surface marker staining emanates from membrane-bound subcellular remnants associated with unrelated cells. Intact macrophages are not present within these cell preparations. The macrophage remnant binding profile reflects interactions between macrophages and other cell types in vivo. Depletion of CD169

Identifiants

DOI: 10.1016/j.celrep.2021.110058 PMID: 34818538
pubmed: 34818538
pii: S2211-1247(21)01544-8
doi: 10.1016/j.celrep.2021.110058
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

110058

Informations de copyright

Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Susan M Millard (SM)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Ostyn Heng (O)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Khatora S Opperman (KS)

Myeloma Research Laboratory, Adelaide Medical School, Faculty of Health and Medical Sciences, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; South Australian Health and Medical Research Institute, PO Box 11060, Adelaide, SA 5001, Australia.

Anuj Sehgal (A)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Katharine M Irvine (KM)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Simranpreet Kaur (S)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia; The University of Queensland, UQ Diamantina Institute, Brisbane, QLD 4102, Australia.

Cheyenne J Sandrock (CJ)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Andy C Wu (AC)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia; TRI Flow Cytometry Suite, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Graham W Magor (GW)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia; Australian Centre for Blood Diseases, Monash University, Melbourne, VIC 3004, Australia.

Lena Batoon (L)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Andrew C Perkins (AC)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia; Australian Centre for Blood Diseases, Monash University, Melbourne, VIC 3004, Australia.

Jacqueline E Noll (JE)

Myeloma Research Laboratory, Adelaide Medical School, Faculty of Health and Medical Sciences, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; South Australian Health and Medical Research Institute, PO Box 11060, Adelaide, SA 5001, Australia.

Andrew C W Zannettino (ACW)

Myeloma Research Laboratory, Adelaide Medical School, Faculty of Health and Medical Sciences, University of Adelaide, North Terrace, Adelaide, SA 5005, Australia; South Australian Health and Medical Research Institute, PO Box 11060, Adelaide, SA 5001, Australia; Central Adelaide Local Health Network, Adelaide, SA 5001, Australia.

David P Sester (DP)

TRI Flow Cytometry Suite, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Jean-Pierre Levesque (JP)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

David A Hume (DA)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Liza J Raggatt (LJ)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Kim M Summers (KM)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.

Allison R Pettit (AR)

Mater Research Institute-The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia. Electronic address: allison.pettit@mater.uq.edu.au.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Moelle osseuse › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Séparation cellulaire › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiogiques du sang › Hématopoïèse › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système hématopoïétique › Cellules de la moelle osseuse › Cellules souches hématopoïétiques › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Phénomènes physiologiques › Homéostasie › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Fragmentation of tissue-resident macrophages...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Fragmentation of tissue-resident macrophages...