Increased connectivity of hiPSC-derived neural networks in multiphase granular hydrogel scaffolds.
3D printing
Hyaluronan
Hydrogel
Microgel
Neural tissue engineering
iPSC
Journal
Bioactive materials
ISSN: 2452-199X
Titre abrégé: Bioact Mater
Pays: China
ID NLM: 101685294
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
25
02
2021
revised:
17
06
2021
accepted:
07
07
2021
entrez:
25
11
2021
pubmed:
26
11
2021
medline:
26
11
2021
Statut:
epublish
Résumé
To reflect human development, it is critical to create a substrate that can support long-term cell survival, differentiation, and maturation. Hydrogels are promising materials for 3D cultures. However, a bulk structure consisting of dense polymer networks often leads to suboptimal microenvironments that impedes nutrient exchange and cell-to-cell interaction. Herein, granular hydrogel-based scaffolds were used to support 3D human induced pluripotent stem cell (hiPSC)-derived neural networks. A custom designed 3D printed toolset was developed to extrude hyaluronic acid hydrogel through a porous nylon fabric to generate hydrogel granules. Cells and hydrogel granules were combined using a weaker secondary gelation step, forming self-supporting cell laden scaffolds. At three and seven days, granular scaffolds supported higher cell viability compared to bulk hydrogels, whereas granular scaffolds supported more neurite bearing cells and longer neurite extensions (65.52 ± 11.59 μm) after seven days compared to bulk hydrogels (22.90 ± 4.70 μm). Long-term (three-month) cultures of clinically relevant hiPSC-derived neural cells in granular hydrogels supported well established neuronal and astrocytic colonies and a high level of neurite extension both inside and beyond the scaffold. This approach is significant as it provides a simple, rapid and efficient way to achieve a tissue-relevant granular structure within hydrogel cultures.
Identifiants
pubmed: 34820576
doi: 10.1016/j.bioactmat.2021.07.008
pii: S2452-199X(21)00341-8
pmc: PMC8586009
doi:
Types de publication
Journal Article
Langues
eng
Pagination
358-372Informations de copyright
© 2021 The Authors.
Références
BMC Dev Biol. 2008 Sep 22;8:90
pubmed: 18808690
Nature. 2017 May 4;545(7652):48-53
pubmed: 28445462
Biomaterials. 2013 Mar;34(8):2005-16
pubmed: 23237515
Biomaterials. 2013 Jan;34(3):704-12
pubmed: 23103154
Biomaterials. 2017 Sep;138:91-107
pubmed: 28554011
Nat Biotechnol. 2009 Mar;27(3):275-80
pubmed: 19252484
Chembiochem. 2006 Mar;7(3):436-40
pubmed: 16456901
Biomaterials. 2009 Oct;30(28):5049-60
pubmed: 19560816
Nat Mater. 2015 Jul;14(7):737-44
pubmed: 26030305
Acta Biomater. 2015 Aug;22:59-69
pubmed: 25917845
Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3185-90
pubmed: 26944080
Biomaterials. 2004 Mar-Apr;25(7-8):1339-48
pubmed: 14643608
Arch Med Sci. 2018 Jun;14(4):910-919
pubmed: 30002710
Macromol Biosci. 2019 Jan;19(1):e1800248
pubmed: 30259658
Adv Sci (Weinh). 2018 Oct 24;6(1):1801076
pubmed: 30643716
Adv Funct Mater. 2020 Oct 22;30(43):
pubmed: 33708028
Nanoscale. 2010 Jan;2(1):35-44
pubmed: 20648362
Dev Neurobiol. 2011 Nov;71(11):1090-101
pubmed: 21739614
Front Cell Neurosci. 2020 Dec 03;14:607399
pubmed: 33362473
Neuroimage. 2015 May 1;111:59-64
pubmed: 25698157
Biomaterials. 2006 Jan;27(3):452-9
pubmed: 16115674
Cell Stem Cell. 2019 Jun 6;24(6):860-876
pubmed: 31173716
Nat Rev Neurosci. 2017 Oct;18(10):573-584
pubmed: 28878372
Biomaterials. 2009 Sep;30(27):4567-72
pubmed: 19500834
Basic Clin Neurosci. 2013 Fall;4(4):282-6
pubmed: 25337358
Acta Biomater. 2011 Sep;7(9):3267-76
pubmed: 21704198
Mol Cells. 2019 Sep 30;42(9):617-627
pubmed: 31564073
J Biomed Mater Res A. 2007 Dec 1;83(3):636-45
pubmed: 17508416
Biomaterials. 2009 Sep;30(27):4695-9
pubmed: 19539367
Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18412-18423
pubmed: 32694205
Sci Rep. 2017 Nov 22;7(1):16033
pubmed: 29167483
Biotechnol Adv. 2020 Sep - Oct;42:107370
pubmed: 30902729
Stem Cells. 2011 Nov;29(11):1886-97
pubmed: 21956892
Biomacromolecules. 2005 Sep-Oct;6(5):2857-65
pubmed: 16153128
Front Cell Neurosci. 2018 Aug 21;12:261
pubmed: 30186118
Nat Rev Mater. 2016 Dec;1(12):
pubmed: 29657852
Acta Biomater. 2012 Jan;8(1):31-40
pubmed: 21920469
Biomaterials. 2019 Apr;200:56-65
pubmed: 30772759
Curr Opin Biotechnol. 2019 Dec;60:1-8
pubmed: 30481603
Biomaterials. 2006 Jan;27(3):497-504
pubmed: 16099038
Biomaterials. 2014 Jun;35(18):4969-85
pubmed: 24674460
Biomaterials. 2006 Apr;27(10):2265-74
pubmed: 16318872
Cold Spring Harb Symp Quant Biol. 2008;73:377-87
pubmed: 19204067
Biomaterials. 2009 Sep;30(26):4325-35
pubmed: 19501901
Acta Biomater. 2019 Mar 1;86:135-147
pubmed: 30660005
Nature. 2013 Sep 19;501(7467):373-9
pubmed: 23995685
Sci Rep. 2017 Sep 25;7(1):12233
pubmed: 28947763
ACS Biomater Sci Eng. 2021 Sep 13;7(9):4269-4281
pubmed: 33591726
Annu Rev Biomed Eng. 2000;2:9-29
pubmed: 11701505
Proc Natl Acad Sci U S A. 2019 Apr 9;116(15):7483-7492
pubmed: 30923117
ACS Appl Mater Interfaces. 2018 Feb 14;10(6):5305-5317
pubmed: 29381329
Acta Biomater. 2019 Mar 1;86:312-322
pubmed: 30610918
Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):13811-6
pubmed: 25114234
Macromol Biosci. 2009 Jan 9;9(1):20-8
pubmed: 18839402
Adv Mater. 2017 Aug;29(32):
pubmed: 28650574
Nat Rev Mater. 2020 Jan;5(1):20-43
pubmed: 34123409
Acta Biomater. 2011 Aug;7(8):3050-9
pubmed: 21550426
Adv Healthc Mater. 2018 Jun;7(12):e1800225
pubmed: 29717823
Biomaterials. 2017 Aug;136:1-11
pubmed: 28505596
Biomaterials. 2018 Feb;156:159-171
pubmed: 29197747
J Vis Exp. 2015 Oct 23;(105):e52970
pubmed: 26555926
J Biomed Mater Res A. 2010 Sep 15;94(4):1162-71
pubmed: 20694983
Cell. 2006 Aug 25;126(4):677-89
pubmed: 16923388
J Tissue Eng Regen Med. 2011 Jan;5(1):69-84
pubmed: 20717888
Small. 2019 Sep;15(39):e1903147
pubmed: 31410986
Front Bioeng Biotechnol. 2020 Nov 06;8:601704
pubmed: 33240868
Acta Biomater. 2013 Feb;9(2):5162-9
pubmed: 22995405
Cell. 2016 May 19;165(5):1238-1254
pubmed: 27118425
Acta Biomater. 2018 Sep 1;77:48-62
pubmed: 30006317
Adv Funct Mater. 2018 Sep 26;28(39):
pubmed: 32595422
Acta Biomater. 2009 Jul;5(6):1884-97
pubmed: 19250891
Biomaterials. 2009 Sep;30(27):4581-9
pubmed: 19501903
Adv Sci (Weinh). 2016 Dec 20;4(2):1600347
pubmed: 28251054
J Biomed Mater Res A. 2017 Mar;105(3):790-805
pubmed: 27798959
Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5803-8
pubmed: 22451909
Sci Adv. 2015 Oct 23;1(9):e1500758
pubmed: 26601312
J Funct Biomater. 2012 Nov 15;3(4):839-63
pubmed: 24955749
Adv Funct Mater. 2015 Mar 4;25(9):1344-1351
pubmed: 26273242
Biophys J. 2008 Nov 1;95(9):4426-38
pubmed: 18658232
Nat Mater. 2017 Feb;16(2):236-243
pubmed: 27798621
J Biomed Mater Res A. 2021 Mar;109(3):300-312
pubmed: 32490587
Biomed Mater. 2020 Jul 20;15(5):055005
pubmed: 32324167
J Mater Chem B. 2017 Jun 7;5(21):3870-3878
pubmed: 28775848
Chemphyschem. 2018 May 22;19(10):1143-1163
pubmed: 29457860
Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):E5039-48
pubmed: 24248365
J Tissue Eng Regen Med. 2019 Mar;13(3):369-384
pubmed: 30550638
Biomacromolecules. 2002 Nov-Dec;3(6):1304-11
pubmed: 12425669
Adv Mater. 2018 Sep;30(39):e1803087
pubmed: 30066374
Biomaterials. 2009 Dec;30(36):6867-78
pubmed: 19775749
Lab Chip. 2016 Nov 15;16(23):4482-4506
pubmed: 27797383
Biomacromolecules. 2007 Apr;8(4):1109-15
pubmed: 17358076
Front Hum Neurosci. 2016 Nov 08;10:566
pubmed: 27877121
Nat Protoc. 2015 Sep;10(9):1362-73
pubmed: 26270395