RLEP LAMP for the laboratory confirmation of leprosy: towards a point-of-care test.

DNA, Bacterial Humans Laboratories Leprosy / diagnosis Molecular Diagnostic Techniques Mycobacterium leprae / genetics Nucleic Acid Amplification Techniques Point-of-Care Testing Real-Time Polymerase Chain Reaction Sensitivity and Specificity
Dry-reagent-based Household contact LAMP Leprosy Mycobacterium leprae NAAT Point-of-care RLEP

Journal

BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551

Informations de publication

Date de publication:
25 Nov 2021
Historique:
received: 01 04 2021
accepted: 02 11 2021
entrez: 26 11 2021
pubmed: 27 11 2021
medline: 30 11 2021
Statut: epublish

Résumé

Nucleic acid-based amplification tests (NAAT), above all (q)PCR, have been applied for the detection of Mycobacterium leprae in leprosy cases and household contacts with subclinical infection. However, their application in the field poses a range of technical challenges. Loop-mediated isothermal amplification (LAMP), as a promising point-of-care NAAT does not require sophisticated laboratory equipment, is easy to perform, and is applicable for decentralized diagnosis at the primary health care level. Among a range of gene targets, the M. leprae specific repetitive element RLEP is regarded as highly sensitive and specific for diagnostic applications.  METHODS: Our group developed and validated a dry-reagent-based (DRB) RLEP LAMP, provided product specifications for customization of a ready-to-use kit (intended for commercial production) and compared it against the in-house prototype. The assays were optimized for application on a Genie

Sections du résumé

BACKGROUND BACKGROUND
Nucleic acid-based amplification tests (NAAT), above all (q)PCR, have been applied for the detection of Mycobacterium leprae in leprosy cases and household contacts with subclinical infection. However, their application in the field poses a range of technical challenges. Loop-mediated isothermal amplification (LAMP), as a promising point-of-care NAAT does not require sophisticated laboratory equipment, is easy to perform, and is applicable for decentralized diagnosis at the primary health care level. Among a range of gene targets, the M. leprae specific repetitive element RLEP is regarded as highly sensitive and specific for diagnostic applications.  METHODS: Our group developed and validated a dry-reagent-based (DRB) RLEP LAMP, provided product specifications for customization of a ready-to-use kit (intended for commercial production) and compared it against the in-house prototype. The assays were optimized for application on a Genie

Identifiants

DOI: 10.1186/s12879-021-06882-2 PMID: 34823479 PMC: PMC8620619
pubmed: 34823479
doi: 10.1186/s12879-021-06882-2
pii: 10.1186/s12879-021-06882-2
pmc: PMC8620619
doi:

Substances chimiques

DNA, Bacterial 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1186

Informations de copyright

© 2021. The Author(s).

Références

PLoS Negl Trop Dis. 2014 Apr 10;8(4):e2655
pubmed: 24722358
PLoS Negl Trop Dis. 2011 Jul;5(7):e1228
pubmed: 21811641
BMC Biotechnol. 2009 Feb 02;9:7
pubmed: 19187544
J Clin Microbiol. 2006 Sep;44(9):3154-9
pubmed: 16954241
Biotechniques. 2015 Apr 01;58(4):189-94
pubmed: 25861931
PLoS Negl Trop Dis. 2008;2(11):e328
pubmed: 18982056
Infect Dis Poverty. 2020 Nov 12;9(1):156
pubmed: 33183339
Pak J Biol Sci. 2014 Jan 15;17(2):151-66
pubmed: 24783797
Nucleic Acids Res. 2000 Jun 15;28(12):E63
pubmed: 10871386
PLoS Negl Trop Dis. 2012;6(4):e1590
pubmed: 22509415
J Infect Dis. 2013 Aug 15;208(4):645-52
pubmed: 23633405
Int J Lepr Other Mycobact Dis. 1968 Jan-Mar;36(1):78-82
pubmed: 4869698
Trop Med Infect Dis. 2018 Oct 01;3(4):
pubmed: 30275432
Clin Infect Dis. 2016 Dec 1;63(11):1412-1420
pubmed: 27558564
BMC Infect Dis. 2012 Jan 18;12:8
pubmed: 22257432
Lepr Rev. 2001 Dec;72(4):449-61
pubmed: 11826481
Mem Inst Oswaldo Cruz. 2012 Dec;107 Suppl 1:55-9
pubmed: 23283454
Expert Rev Mol Diagn. 2018 Dec;18(12):1063-1074
pubmed: 30381977
Int J Infect Dis. 2013 Nov;17(11):e1005-10
pubmed: 23871279
Sci Rep. 2016 Nov 09;6:36808
pubmed: 27827432
Sci Rep. 2019 Nov 13;9(1):16675
pubmed: 31723144
Mil Med. 2016 May;181(5 Suppl):227-31
pubmed: 27168577
FEMS Immunol Med Microbiol. 2009 Jan;55(1):1-5
pubmed: 19040664
Clin Microbiol Infect. 2014 May;20(5):447-52
pubmed: 24033793
J Virol Methods. 2017 Jun;244:32-38
pubmed: 28242293
J Appl Microbiol. 2018 Mar;124(3):626-643
pubmed: 29165905
Nat Rev Microbiol. 2004 Mar;2(3):231-40
pubmed: 15083158
Expert Rev Mol Diagn. 2012 Sep;12(7):687-701
pubmed: 23153237
Indian J Dermatol Venereol Leprol. 2021 [SEASON];87(4):491-497
pubmed: 34219438
Expert Rev Mol Diagn. 2020 May;20(5):455-465
pubmed: 32116067
Parasit Vectors. 2015 Oct 22;8:548
pubmed: 26490878
Lancet Infect Dis. 2014 Feb;14(2):96-8
pubmed: 24457165
Lancet Glob Health. 2021 Jan;9(1):e81-e90
pubmed: 33129378
PLoS Negl Trop Dis. 2012;6(8):e1756
pubmed: 22953006
PLoS Negl Trop Dis. 2017 Jan 23;11(1):e0005325
pubmed: 28114411
Methods. 2010 Apr;50(4):S1-5
pubmed: 20215014
Int J Lepr Other Mycobact Dis. 2004 Sep;72(3):269-77
pubmed: 15485283
J Clin Microbiol. 2018 Feb 22;56(3):
pubmed: 29305545
J Clin Microbiol. 2016 Aug;54(8):1984-91
pubmed: 27194691
FEMS Immunol Med Microbiol. 2008 Nov;54(2):263-70
pubmed: 18783434
Int J Lepr Other Mycobact Dis. 1993 Sep;61(3):389-93
pubmed: 8228436
BMC Infect Dis. 2010 Aug 24;10:252
pubmed: 20735843
PLoS Negl Trop Dis. 2012;6(6):e1572
pubmed: 22745836
J Infect Dis. 2013 Aug 15;208(4):637-44
pubmed: 23633403
Mol Microbiol. 1990 Oct;4(10):1745-51
pubmed: 2077358
Am J Trop Med Hyg. 2021 Apr 19;104(6):2085-2090
pubmed: 33872215
PLoS Negl Trop Dis. 2018 Feb 14;12(2):e0006264
pubmed: 29444079
Nucleic Acids Res. 2009 Jan;37(Database issue):D26-31
pubmed: 18940867
PLoS Negl Trop Dis. 2013;7(1):e2011
pubmed: 23359828
Biosens Bioelectron. 2014 Nov 15;61:491-9
pubmed: 24949822
Int J Mycobacteriol. 2015 Mar;4(1):54-9
pubmed: 26655199
Infection. 2019 Dec;47(6):1065-1069
pubmed: 31456174
BMC Infect Dis. 2019 Aug 28;19(1):753
pubmed: 31462296
Parasit Vectors. 2017 Jan 13;10(1):26
pubmed: 28086864
Diagn Microbiol Infect Dis. 2003 Sep;47(1):349-58
pubmed: 12967749
Sci Rep. 2019 Oct 14;9(1):14744
pubmed: 31611563
PLoS Negl Trop Dis. 2018 Apr 12;12(4):e0006396
pubmed: 29649256
Acta Trop. 2019 Sep;197:105041
pubmed: 31152726
PLoS Negl Trop Dis. 2015 Mar 13;9(3):e0003578
pubmed: 25769046
J Clin Microbiol. 2017 Oct;55(10):3037-3045
pubmed: 28768730
Int J Infect Dis. 2020 Sep;98:408-419
pubmed: 32659450
Transbound Emerg Dis. 2017 Jun;64(3):861-871
pubmed: 26617330
PLoS Negl Trop Dis. 2015 Nov 13;9(11):e0004219
pubmed: 26566026
J Microbiol. 2015 Jan;53(1):1-5
pubmed: 25557475
Int J Infect Dis. 2021 Jun;107:145-152
pubmed: 33864913
Int J Lepr Other Mycobact Dis. 1994 Mar;62(1):1-9
pubmed: 8189074
Appl Environ Microbiol. 2007 Aug;73(15):4733-40
pubmed: 17526786
Diagn Microbiol Infect Dis. 2019 Nov;95(3):114855
pubmed: 31285121
An Bras Dermatol. 2014 Mar-Apr;89(2):205-18
pubmed: 24770495
Clin Infect Dis. 2007 Apr 15;44(8):1096-9
pubmed: 17366457
PLoS Negl Trop Dis. 2011 Oct;5(10):e1354
pubmed: 22022631
Infect Dis Poverty. 2019 Mar 25;8(1):20
pubmed: 30905322
Vet Parasitol. 2018 Aug 30;260:53-57
pubmed: 30197015
PLoS Negl Trop Dis. 2019 Nov 7;13(11):e0007698
pubmed: 31697673
Jpn J Infect Dis. 2012;65(3):247-51
pubmed: 22627308
PLoS Negl Trop Dis. 2008 Feb 06;2(1):e147
pubmed: 18253475
Parasit Vectors. 2018 Apr 17;11(1):250
pubmed: 29665825
Bull World Health Organ. 2012 Dec 1;90(12):914-20
pubmed: 23284197
PLoS Negl Trop Dis. 2019 Jun 10;13(6):e0007400
pubmed: 31181059
Sci Rep. 2021 May 10;11(1):9859
pubmed: 33972644
Acta Trop. 2018 Jun;182:223-231
pubmed: 29545156

Auteurs

Malkin Saar (M)

Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University (LMU) Munich, Leopoldstrasse 5, 80802, Munich, Germany. Malkin.Saar@lrz.uni-muenchen.de.
ORCID: 0000-0002-8027-1892

Marcus Beissner (M)

Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University (LMU) Munich, Leopoldstrasse 5, 80802, Munich, Germany.
ORCID: 0000-0001-9238-2998

Fatih Gültekin (F)

Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University (LMU) Munich, Leopoldstrasse 5, 80802, Munich, Germany.

Issaka Maman (I)

Ministère de la Santé, Institut National d'Hygiène (INH), Lomé, Togo.

Karl-Heinz Herbinger (KH)

Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University (LMU) Munich, Leopoldstrasse 5, 80802, Munich, Germany.

Gisela Bretzel (G)

Division of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians-University (LMU) Munich, Leopoldstrasse 5, 80802, Munich, Germany.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN bactérien › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Établissements, main d'oeuvre et services de soins de santé › Établissements de santé › Laboratoires › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à Actinomycetales › Infections à Mycobacterium › Infections à mycobactéries non tuberculeuses › Lèpre › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de diagnostic moléculaire › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Administration des services de santé › Gestion des soins aux patients › Systèmes automatisés lit malade › Analyse sur le lieu d'intervention › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › RLEP LAMP for the laboratory confirmation of...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › RLEP LAMP for the laboratory confirmation of...