Antiviral Effect of Lithium Chloride on Replication of Marek's Disease Virus in Chicken Embryonic Fibroblasts.
2',5'-Oligoadenylate Synthetase
/ genetics
Animals
Antiviral Agents
/ pharmacology
Cell Line
Chick Embryo
Chickens
Dose-Response Relationship, Drug
Fibroblasts
/ drug effects
Gene Expression Regulation
Herpesvirus 2, Gallid
/ drug effects
Host-Pathogen Interactions
/ drug effects
Interferon Regulatory Factor-7
/ genetics
Interferon-beta
/ genetics
Lithium Chloride
/ pharmacology
Oncogene Proteins, Viral
/ antagonists & inhibitors
Viral Load
/ drug effects
Virus Replication
/ drug effects
Marek’s disease virus
inhibition
lithium chloride
virus replication
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Nov 2021
16 Nov 2021
Historique:
received:
10
10
2021
revised:
09
11
2021
accepted:
15
11
2021
entrez:
27
11
2021
pubmed:
28
11
2021
medline:
17
12
2021
Statut:
epublish
Résumé
To investigate the antiviral effect of lithium chloride (LiCl) on the replication of Marek's disease virus (MDV) in chicken embryonic fibroblast (CEF) cells, real-time PCR, Western blotting, plaque counting, and indirect immunofluorescence experiments were performed at different time points of LiCl treated CEF cells with virus infection. The results demonstrated that LiCl could affect multiple steps of virus replication and inhibit viral gene expression and protein synthesis in a dose- and time-dependent manner. Moreover, LiCl could directly affect viral infectivity as well. In addition, LiCl significantly affected the gene expression of IFN-β related genes in virus-infected cells. These results indicate that LiCl significantly inhibits MDV replication and proliferation in CEF cells and it has the potential to be used as an antiviral agent against MDV.
Identifiants
pubmed: 34830257
pii: ijms222212375
doi: 10.3390/ijms222212375
pmc: PMC8623539
pii:
doi:
Substances chimiques
Antiviral Agents
0
Eco-Q protein, Gallid herpesvirus 2
0
Interferon Regulatory Factor-7
0
Oncogene Proteins, Viral
0
Interferon-beta
77238-31-4
2',5'-Oligoadenylate Synthetase
EC 2.7.7.84
Lithium Chloride
G4962QA067
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 31772734; 32072839
Organisme : Jiangsu Agricultural independent innovation pro-ject
ID : CX(19)3008
Organisme : NCFC-RCUK-BBSRC
ID : 31761133002
Organisme : BSRC Newton Fund
ID : BB/R012865/1
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