Aquaporin-1 Facilitates Transmesothelial Water Permeability: In Vitro and Ex Vivo Evidence and Possible Implications in Peritoneal Dialysis.
aquaporin 1
mesothelial cells
peritoneal dialysis
three-pore model
water transport
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 Nov 2021
21 Nov 2021
Historique:
received:
22
10
2021
accepted:
18
11
2021
entrez:
27
11
2021
pubmed:
28
11
2021
medline:
18
12
2021
Statut:
epublish
Résumé
We previously showed that mesothelial cells in human peritoneum express the water channel aquaporin 1 (AQP1) at the plasma membrane, suggesting that, although in a non-physiological context, it may facilitate osmotic water exchange during peritoneal dialysis (PD). According to the three-pore model that predicts the transport of water during PD, the endothelium of peritoneal capillaries is the major limiting barrier to water transport across peritoneum, assuming the functional role of the mesothelium, as a semipermeable barrier, to be negligible. We hypothesized that an intact mesothelial layer is poorly permeable to water unless AQP1 is expressed at the plasma membrane. To demonstrate that, we characterized an immortalized cell line of human mesothelium (HMC) and measured the osmotically-driven transmesothelial water flux in the absence or in the presence of AQP1. The presence of tight junctions between HMC was investigated by immunofluorescence. Bioelectrical parameters of HMC monolayers were studied by Ussing Chambers and transepithelial water transport was investigated by an electrophysiological approach based on measurements of TEA
Identifiants
pubmed: 34830416
pii: ijms222212535
doi: 10.3390/ijms222212535
pmc: PMC8622642
pii:
doi:
Substances chimiques
AQP1 protein, human
0
Aquaporins
0
Aquaporin 1
146410-94-8
Sodium
9NEZ333N27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : MIUR
ID : Research and Innovation PON 2014 - 2020, Action I.1 "Innovative PhDs with industrial charac-terization", project title "Innovative biocompatible hypertonic solutions for peritoneal dialysis"
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