Process Development for Newcastle Disease Virus-Vectored Vaccines in Serum-Free Vero Cell Suspension Cultures.

COVID-19 Newcastle Disease Virus SARS-CoV-2 Vero suspension culture bioreactor production vaccine production platform viral vaccine bioprocess

Journal

Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355

Informations de publication

Date de publication:
16 Nov 2021
Historique:
received: 19 10 2021
revised: 11 11 2021
accepted: 12 11 2021
entrez: 27 11 2021
pubmed: 28 11 2021
medline: 28 11 2021
Statut: epublish

Résumé

The ongoing COVID-19 pandemic drew global attention to infectious diseases, attracting numerous resources for development of pandemic preparedness plans and vaccine platforms-technologies with robust manufacturing processes that can quickly be pivoted to target emerging diseases. Newcastle Disease Virus (NDV) has been studied as a viral vector for human and veterinary vaccines, but its production relies heavily on embryonated chicken eggs, with very few studies producing NDV in cell culture. Here, NDV is produced in suspension Vero cells, and analytical assays (TCID

Identifiants

pubmed: 34835266
pii: vaccines9111335
doi: 10.3390/vaccines9111335
pmc: PMC8623276
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Canada Research Chairs
ID : CRC-240394
Organisme : National Research Council of Canada
ID : PR-013.1
Organisme : Mitacs
ID : Globalink Fellowship
Organisme : Fonds de Recherche du Québec - Santé
ID : Award

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Auteurs

Julia Puppin Chaves Fulber (JPC)

Viral Vectors and Vaccines Bioprocessing Group, Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.

Omar Farnós (O)

Viral Vectors and Vaccines Bioprocessing Group, Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.

Sascha Kiesslich (S)

Viral Vectors and Vaccines Bioprocessing Group, Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.

Zeyu Yang (Z)

Viral Vectors and Vaccines Bioprocessing Group, Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.

Shantoshini Dash (S)

Viral Vectors and Vaccines Bioprocessing Group, Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.

Leonardo Susta (L)

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Sarah K Wootton (SK)

Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Amine A Kamen (AA)

Viral Vectors and Vaccines Bioprocessing Group, Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.

Classifications MeSH